Title |
Zika Virus Infects Human Cortical Neural Progenitors and Attenuates Their Growth
|
---|---|
Published in |
Cell Stem Cell, March 2016
|
DOI | 10.1016/j.stem.2016.02.016 |
Pubmed ID | |
Authors |
Hengli Tang, Christy Hammack, Sarah C. Ogden, Zhexing Wen, Xuyu Qian, Yujing Li, Bing Yao, Jaehoon Shin, Feiran Zhang, Emily M. Lee, Kimberly M. Christian, Ruth A. Didier, Peng Jin, Hongjun Song, Guo-li Ming |
Abstract |
The suspected link between infection by Zika virus (ZIKV), a re-emerging flavivirus, and microcephaly is an urgent global health concern. The direct target cells of ZIKV in the developing human fetus are not clear. Here we show that a strain of the ZIKV, MR766, serially passaged in monkey and mosquito cells efficiently infects human neural progenitor cells (hNPCs) derived from induced pluripotent stem cells. Infected hNPCs further release infectious ZIKV particles. Importantly, ZIKV infection increases cell death and dysregulates cell-cycle progression, resulting in attenuated hNPC growth. Global gene expression analysis of infected hNPCs reveals transcriptional dysregulation, notably of cell-cycle-related pathways. Our results identify hNPCs as a direct ZIKV target. In addition, we establish a tractable experimental model system to investigate the impact and mechanism of ZIKV on human brain development and provide a platform to screen therapeutic compounds. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 63 | 23% |
United Kingdom | 20 | 7% |
Japan | 14 | 5% |
France | 8 | 3% |
Canada | 7 | 3% |
Netherlands | 5 | 2% |
Colombia | 4 | 1% |
Australia | 3 | 1% |
Germany | 3 | 1% |
Other | 39 | 14% |
Unknown | 103 | 38% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 185 | 69% |
Scientists | 60 | 22% |
Science communicators (journalists, bloggers, editors) | 14 | 5% |
Practitioners (doctors, other healthcare professionals) | 10 | 4% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Brazil | 12 | <1% |
United States | 10 | <1% |
France | 3 | <1% |
United Kingdom | 2 | <1% |
Korea, Republic of | 1 | <1% |
Italy | 1 | <1% |
Switzerland | 1 | <1% |
South Africa | 1 | <1% |
Hong Kong | 1 | <1% |
Other | 5 | <1% |
Unknown | 1332 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 248 | 18% |
Student > Ph. D. Student | 222 | 16% |
Researcher | 191 | 14% |
Student > Master | 182 | 13% |
Student > Doctoral Student | 85 | 6% |
Other | 216 | 16% |
Unknown | 225 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 286 | 21% |
Biochemistry, Genetics and Molecular Biology | 242 | 18% |
Medicine and Dentistry | 180 | 13% |
Immunology and Microbiology | 108 | 8% |
Neuroscience | 94 | 7% |
Other | 185 | 14% |
Unknown | 274 | 20% |