↓ Skip to main content

Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins

Overview of attention for article published in Microbial Cell Factories, April 2015
Altmetric Badge

Mentioned by

twitter
1 tweeter
facebook
1 Facebook page

Citations

dimensions_citation
162 Dimensions

Readers on

mendeley
356 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins
Published in
Microbial Cell Factories, April 2015
DOI 10.1186/s12934-015-0241-5
Pubmed ID
Authors

Uwe Mamat, Kathleen Wilke, David Bramhill, Andra Beate Schromm, Buko Lindner, Thomas Andreas Kohl, José Luis Corchero, Antonio Villaverde, Lana Schaffer, Steven Robert Head, Chad Souvignier, Timothy Charles Meredith, Ronald Wesley Woodard

Abstract

Lipopolysaccharide (LPS), also referred to as endotoxin, is the major constituent of the outer leaflet of the outer membrane of virtually all Gram-negative bacteria. The lipid A moiety, which anchors the LPS molecule to the outer membrane, acts as a potent agonist for Toll-like receptor 4/myeloid differentiation factor 2-mediated pro-inflammatory activity in mammals and, thus, represents the endotoxic principle of LPS. Recombinant proteins, commonly manufactured in Escherichia coli, are generally contaminated with endotoxin. Removal of bacterial endotoxin from recombinant therapeutic proteins is a challenging and expensive process that has been necessary to ensure the safety of the final product. As an alternative strategy for common endotoxin removal methods, we have developed a series of E. coli strains that are able to grow and express recombinant proteins with the endotoxin precursor lipid IVA as the only LPS-related molecule in their outer membranes. Lipid IVA does not trigger an endotoxic response in humans typical of bacterial LPS chemotypes. Hence the engineered cells themselves, and the purified proteins expressed within these cells display extremely low endotoxin levels. This paper describes the preparation and characterization of endotoxin-free E. coli strains, and demonstrates the direct production of recombinant proteins with negligible endotoxin contamination.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 356 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 <1%
Spain 1 <1%
China 1 <1%
Canada 1 <1%
Unknown 352 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 80 22%
Student > Bachelor 63 18%
Student > Master 53 15%
Researcher 49 14%
Student > Doctoral Student 14 4%
Other 27 8%
Unknown 70 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 104 29%
Agricultural and Biological Sciences 71 20%
Chemistry 19 5%
Immunology and Microbiology 18 5%
Engineering 17 5%
Other 43 12%
Unknown 84 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2016.
All research outputs
#9,394,668
of 12,247,570 outputs
Outputs from Microbial Cell Factories
#597
of 893 outputs
Outputs of similar age
#186,600
of 287,176 outputs
Outputs of similar age from Microbial Cell Factories
#19
of 29 outputs
Altmetric has tracked 12,247,570 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 893 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 287,176 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.