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Cell-Based Assays Using iPSCs for Drug Development and Testing

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Cover of 'Cell-Based Assays Using iPSCs for Drug Development and Testing'

Table of Contents

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    Book Overview
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    Chapter 1 Cell-Based Assays Using Differentiated Human Induced Pluripotent Cells
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    Chapter 2 Monolayer Generation of Vascular Endothelial Cells from Human Pluripotent Stem Cells
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    Chapter 3 Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling
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    Chapter 4 Human Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic Differentiation
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    Chapter 5 Production of Cardiomyocytes from Human Pluripotent Stem Cells by Bioreactor Technologies
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    Chapter 6 Improved Protocol for Cardiac Differentiation and Maturation of Pluripotent Stem Cells
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    Chapter 7 Human Pluripotent Stem Cell Expansion in Stirred Tank Bioreactors
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    Chapter 8 A Protocol for One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature Podocytes
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    Chapter 9 Differentiation of hiPS Cells into Definitive Endoderm for High-Throughput Screening
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    Chapter 10 LC-MS/MS-Based Metabolomics for Cell Cultures
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    Chapter 11 Preparation of iPSCs for Targeted Proteomic Analysis
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    Chapter 12 Measurement of Bile Acids as a Marker of the Functionality of iPSC-Derived Hepatocytes
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    Chapter 13 Mass Spectrometry Measurement of Albumin–Alpha Fetoprotein Ratio as an Indicator of iPSC-Derived Hepatocyte Differentiation
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    Chapter 14 Seeding Induced Pluripotent Stem Cell-Derived Neurons onto 384-Well Plates
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    Chapter 15 High-Content Autophagy Analysis in iPSC-Derived Neurons Using Immunofluorescence
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    Chapter 16 High-Content Analysis of Mitochondrial Function in iPSC-Derived Neurons
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    Chapter 17 Evaluating the Effect of Drug Compounds on Cardiac Spheroids Using the Cardiac Cell Outgrowth Assay
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    Chapter 18 Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes Using Multiwell Microelectrode Arrays
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    Chapter 19 Multielectrode Array (MEA)-Based Detection of Spontaneous Network Activity in Human iPSC-Derived Cortical Neurons
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    Chapter 20 Assessing Neuronal Excitability on a Fluorometric Imaging Plate Reader (FLIPR) Following a Defined Electrostimulation Paradigm
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    Chapter 21 Fabrication of a Microfluidic Cell Culture Device Using Photolithographic and Soft Lithographic Techniques
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    Chapter 22 Using a Microfluidic Device for Culture and Drug Toxicity Testing of 3D Cells
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    Chapter 23 hiPS Cell-Derived Neurons for High-Throughput Screening
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