Antigen Processing

Table of Contents

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   Book Overview
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   Chapter 1 Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes
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   Chapter 2 Analysis of Proteasome-Generated Antigenic Peptides by Mass Spectrometry
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   Chapter 3 Trimming of MHC Class I Ligands by ERAP Aminopeptidases
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   Chapter 4 Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases
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   Chapter 5 Testing the Impact of Protease Inhibitors in Antigen Presentation Assays
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   Chapter 6 In Vitro Studies of MHC Class I Peptide Loading and Exchange
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   Chapter 7 Measuring Synthesis and Degradation of MHC Class I Molecules
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   Chapter 8 Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules
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    Chapter 9 A SIINFEKL-Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics
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    Chapter 10 Purification and Identification of Naturally Presented MHC Class I and II Ligands
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    Chapter 11 Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs
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    Chapter 12 Quantitating MHC Class I Ligand Production and Presentation Using TCR-Like Antibodies
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    Chapter 13 Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors
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    Chapter 14 A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway
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    Chapter 15 Evaluating CD8+ T Cell Responses In Vitro
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    Chapter 16 Recombinant Poxviruses: Versatile Tools for Immunological Assays
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    Chapter 17 Monitoring Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy
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    Chapter 18 Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases
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    Chapter 19 Monitoring MHC-II Endocytosis and Recycling Using Cell-Surface Protein Biotinylation-Based Assays
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    Chapter 20 Monitoring Protein Endocytosis and Recycling Using FACS-Based Assays
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    Chapter 21 In Vitro Digestion with Proteases Producing MHC Class II Ligands
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    Chapter 22 Assembly, Intracellular Transport, and Release of MHC Class II Peptide Receptors
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    Chapter 23 Pulse–Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading
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    Chapter 24 Studying MHC Class II Peptide Loading and Editing In Vitro
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    Chapter 25 Monitoring Antigen Processing for MHC Presentation via Macroautophagy
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    Chapter 26 Identification of Human Antigen-Specific CD4+ T-Cells with Peptide–MHC Multimer Technologies
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    Chapter 27 Production of Antigen-Specific Human CD4+ T Cell Lines and Clones
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    Chapter 28 Monitoring Dendritic Cell Activation and Maturation
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    Chapter 29 Studying MHC Class II Presentation of Immobilized Antigen by B Lymphocytes
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    Chapter 30 Analyzing Antigen Recognition by Natural Killer T Cells