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Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

Overview of attention for article published in Brazilian Oral Research, January 2016
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Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm
Published in
Brazilian Oral Research, January 2016
DOI 10.1590/1807-3107bor-2016.vol30.0038
Pubmed ID

REZENDE, Gabriely Cristinni, MASSUNARI, Loiane, QUEIROZ, India Olinta de Azevedo, GOMES FILHO, João Eduardo, JACINTO, Rogério Castilho, LODI, Carolina Simonetti, DEZAN JUNIOR, Elói, Rezende, Gabriely Cristinni, Massunari, Loiane, Queiroz, India Olinta de Azevedo, Gomes Filho, João Eduardo, Jacinto, Rogério Castilho, Lodi, Carolina Simonetti, Dezan, Elói


Enterococcus faecalis are gram positive bacteria that can mostly resist endodontic therapy, inducing persistent infection in the root canal system. Endodontic sealers with antimicrobial activity may help eliminate residual microorganisms that survive endodontic treatment. The present study aimed at comparing the antimicrobial activity of Acroseal, Sealapex and AH Plus endodontic sealers in an in vitro biofilm model. Bovine dentin specimens (144) were prepared, and twelve blocks for each sealer and each experimental time point (2, 7 and 14 days) were placed and left in contact with plates containing inoculum of E. faecalis (ATCC 51299), to induce biofilm formation. After 14 days, the samples were transferred to another plate with test sealers and kept at 37°C and 5% CO2 for 2, 7 and 14 days. The specimens without sealers were used as a control for each period. The samples were agitated in a sonicator after each experiment. The suspensions were agitated in a vortex mixer, serially diluted in saline, and triple plated onto m-Enterococcus agar. Colonyforming units were counted, and the data were statistically analyzed using ANOVA, Shapiro-Wilk and Kruskal-Wallis one-way tests (p < 0.05) to determine antimicrobial potential. Sealapex showed significant differences at all the experimental time points, in comparison with all the other groups. AH Plus and Acroseal showed antimicrobial activity only on the 14th experimental day. Neither of the sealers tested were able to completely eliminate the biofilm. Sealapex showed the highest antimicrobial activity in all the experimental periods. The antimicrobial activity of all the sealers analyzed increased over time.

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Geographical breakdown

Country Count As %
Unknown 53 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 13%
Student > Postgraduate 6 11%
Student > Ph. D. Student 6 11%
Researcher 4 8%
Student > Bachelor 4 8%
Other 8 15%
Unknown 18 34%
Readers by discipline Count As %
Medicine and Dentistry 23 43%
Agricultural and Biological Sciences 3 6%
Immunology and Microbiology 2 4%
Veterinary Science and Veterinary Medicine 1 2%
Biochemistry, Genetics and Molecular Biology 1 2%
Other 2 4%
Unknown 21 40%