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Sperm cryodamage occurs after rapid freezing phase: flow cytometry approach and antioxidant enzymes activity at different stages of cryopreservation

Overview of attention for article published in Journal of Animal Science and Biotechnology, March 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#29 of 273)
  • Good Attention Score compared to outputs of the same age (76th percentile)

Mentioned by

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11 tweeters

Citations

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19 Dimensions

Readers on

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53 Mendeley
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Title
Sperm cryodamage occurs after rapid freezing phase: flow cytometry approach and antioxidant enzymes activity at different stages of cryopreservation
Published in
Journal of Animal Science and Biotechnology, March 2016
DOI 10.1186/s40104-016-0076-x
Pubmed ID
Authors

L. S. Castro, T. R. S. Hamilton, C. M. Mendes, M. Nichi, V. H. Barnabe, J. A. Visintin, M. E. O. A. Assumpção

Abstract

In order to improve the efficiency of bovine sperm cryopreservation process, it is important to understand how spermatozoa respond to differences in temperature as well as the ability to recover its own metabolism. The combination between flow cytometry approach and antioxidant enzymes activity allows a more sensible evaluation of sperm cell during cryopreservation. The aim of this study was to evaluate sperm attributes and antioxidant enzymes activity during different stages of cryopreservation process. Semen samples from Holstein bulls (n = 4) were separated in 3 treatments: fresh (37 °C); cooled (5 °C); and thawed. Evaluation occurred at 0 h and 2 h after incubation. Membrane integrity, mitochondrial membrane potential (MMP) and DNA damages were evaluated by flow cytometry; activities of antioxidant enzymes such as catalase, superoxide dismutase and gluthatione peroxidase were measured by spectrofotometry. There was an increase in the percentage of sperm with DNA damage in the thawed group, compared to fresh and cooled, and for 2 hs of incubation when compared to 0 h. Considering MMP, there was an increase in the percentage of cells with medium potential in thawed group when compared to fresh and cooled groups. Opposingly, a decrease was observed in the thawed group considering high mitochondrial potential. Also in the thawed group, there was an increase on cells with damaged acrosome and membrane when compared to fresh and cooled groups. Significant correlations were found between antioxidant enzymes activity and membrane or mitochondrial parameters. Based on our results, we conclude that cryopreservation affects cellular and DNA integrity and that the critical moment is when sperm cells are exposed to freezing temperature. Also, our study indicates that intracellular antioxidant machinery (SOD and GPX enzymes) is not enough to control cryodamage.

Twitter Demographics

The data shown below were collected from the profiles of 11 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 53 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 10 19%
Student > Master 9 17%
Student > Ph. D. Student 8 15%
Researcher 6 11%
Student > Doctoral Student 5 9%
Other 7 13%
Unknown 8 15%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 16 30%
Agricultural and Biological Sciences 13 25%
Biochemistry, Genetics and Molecular Biology 3 6%
Medicine and Dentistry 3 6%
Immunology and Microbiology 1 2%
Other 3 6%
Unknown 14 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 March 2016.
All research outputs
#2,746,909
of 12,554,960 outputs
Outputs from Journal of Animal Science and Biotechnology
#29
of 273 outputs
Outputs of similar age
#62,906
of 266,487 outputs
Outputs of similar age from Journal of Animal Science and Biotechnology
#1
of 4 outputs
Altmetric has tracked 12,554,960 research outputs across all sources so far. Compared to these this one has done well and is in the 78th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 273 research outputs from this source. They receive a mean Attention Score of 2.5. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 266,487 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them