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A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)

Overview of attention for article published in BMC Plant Biology, March 2016
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Title
A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)
Published in
BMC Plant Biology, March 2016
DOI 10.1186/s12870-016-0751-2
Pubmed ID
Authors

Annick Bertrand, Marie Bipfubusa, Yves Castonguay, Solen Rocher, Aleksandra Szopinska-Morawska, Yousef Papadopoulos, Jenny Renaut

Abstract

Improvement of freezing tolerance of red clover (Trifolium pratense L.) would increase its persistence under cold climate. In this study, we assessed the freezing tolerance and compared the proteome composition of non-acclimated and cold-acclimated plants of two initial cultivars of red clover: Endure (E-TF0) and Christie (C-TF0) and of populations issued from these cultivars after three (TF3) and four (TF4) cycles of phenotypic recurrent selection for superior freezing tolerance. Through this approach, we wanted to identify proteins that are associated with the improvement of freezing tolerance in red clover. Freezing tolerance expressed as the lethal temperature for 50 % of the plants (LT50) increased markedly from approximately -2 to -16 °C following cold acclimation. Recurrent selection allowed a significant 2 to 3 °C increase of the LT50 after four cycles of recurrent selection. Two-dimensional difference gel electrophoresis (2D-DIGE) was used to study variations in protein abundance. Principal component analysis based on 2D-DIGE revealed that the largest variability in the protein data set was attributable to the cold acclimation treatment and that the two genetic backgrounds had differential protein composition in the acclimated state only. Vegetative storage proteins (VSP), which are essential nitrogen reserves for plant regrowth, and dehydrins were among the most striking changes in proteome composition of cold acclimated crowns of red clovers. A subset of proteins varied in abundance in response to selection including a dehydrin that increased in abundance in TF3 and TF4 populations as compared to TF0 in the Endure background. Recurrent selection performed indoor is an effective approach to improve the freezing tolerance of red clover. Significant improvement of freezing tolerance by recurrent selection was associated with differential accumulation of a small number of cold-regulated proteins that may play an important role in the determination of the level of freezing tolerance.

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Geographical breakdown

Country Count As %
Unknown 33 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 21%
Student > Master 5 15%
Researcher 4 12%
Student > Bachelor 3 9%
Other 2 6%
Other 5 15%
Unknown 7 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 45%
Biochemistry, Genetics and Molecular Biology 6 18%
Veterinary Science and Veterinary Medicine 1 3%
Environmental Science 1 3%
Economics, Econometrics and Finance 1 3%
Other 1 3%
Unknown 8 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 March 2016.
All research outputs
#20,315,221
of 22,856,968 outputs
Outputs from BMC Plant Biology
#2,521
of 3,257 outputs
Outputs of similar age
#253,437
of 300,113 outputs
Outputs of similar age from BMC Plant Biology
#55
of 66 outputs
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