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Optogenetics

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Cover of 'Optogenetics'

Table of Contents

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    Book Overview
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    Chapter 1 Optogenetics
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    Chapter 2 Optogenetics
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    Chapter 3 Optogenetics
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    Chapter 4 Reversible Photoregulation of Gene Expression and Translation
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    Chapter 5 Controlling Protein Activity and Degradation Using Blue Light.
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    Chapter 6 Photo Control of Protein Function Using Photoactive Yellow Protein
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    Chapter 7 Optogenetics
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    Chapter 8 Optogenetic Control of Pancreatic Islets
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    Chapter 9 Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
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    Chapter 10 Optogenetics
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    Chapter 11 Optogenetics
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    Chapter 12 Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches
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    Chapter 13 Optogenetic Modulation of Locomotor Activity on Free-Behaving Rats
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    Chapter 14 Combined Optogenetic and Chemogenetic Control of Neurons
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    Chapter 15 Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex
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    Chapter 16 An Optimized Calcium-Phosphate Transfection Method for Characterizing Genetically Encoded Tools in Primary Neurons
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    Chapter 17 Optogenetic Approaches for Mesoscopic Brain Mapping
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    Chapter 18 Optogenetic Tools for Confined Stimulation in Deep Brain Structures
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    Chapter 19 Optogenetics
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    Chapter 20 Optogenetic Light Crafting Tools for the Control of Cardiac Arrhythmias
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    Chapter 21 Inscribing Optical Excitability to Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary Cardiac Fibroblasts
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    Chapter 22 Optogenetic Engineering of Atrial Cardiomyocytes
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    Chapter 23 A Multichannel Recording System with Optical Stimulation for Closed-Loop Optogenetic Experiments
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    Chapter 24 Optogenetic Control of Fibroblast Growth Factor Receptor Signaling
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    Chapter 25 Protein Inactivation by Optogenetic Trapping in Living Cells
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    Chapter 26 Optogenetics
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    Chapter 27 Guidelines for Photoreceptor Engineering
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    Chapter 28 Erratum to: Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
Attention for Chapter 25: Protein Inactivation by Optogenetic Trapping in Living Cells
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Chapter title
Protein Inactivation by Optogenetic Trapping in Living Cells
Chapter number 25
Book title
Optogenetics
Published in
Methods in molecular biology, March 2016
DOI 10.1007/978-1-4939-3512-3_25
Pubmed ID
Book ISBNs
978-1-4939-3510-9, 978-1-4939-3512-3
Authors

Hyerim Park, Sangkyu Lee, Won Do Heo

Editors

Arash Kianianmomeni

Abstract

Optogenetic modules that use genetically encoded elements to control protein function in response to light allow for precise spatiotemporal modulation of signaling pathways. As one of optical approaches, LARIAT (Light-Activated Reversible Inhibition by Assembled Trap) is a unique light-inducible inhibition system that reversibly sequesters target proteins into clusters, generated by multimeric proteins and a blue light-induced heterodimerization module. Here we present a method based on LARIAT for optical inhibition of targets in living mammalian cells. In the protocol, we focus on the inhibition of proteins that modulate cytoskeleton and cell cycle, and describe how to transfect, conduct a photo-stimulation, and analyze the data.

Mendeley readers

The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 69 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 14 20%
Student > Ph. D. Student 13 19%
Student > Bachelor 7 10%
Student > Doctoral Student 6 9%
Professor > Associate Professor 6 9%
Other 14 20%
Unknown 9 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 24 35%
Agricultural and Biological Sciences 15 22%
Chemistry 6 9%
Engineering 4 6%
Immunology and Microbiology 2 3%
Other 8 12%
Unknown 10 14%