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Optogenetics

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Cover of 'Optogenetics'

Table of Contents

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    Book Overview
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    Chapter 1 Optogenetics
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    Chapter 2 Optogenetics
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    Chapter 3 Optogenetics
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    Chapter 4 Reversible Photoregulation of Gene Expression and Translation
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    Chapter 5 Controlling Protein Activity and Degradation Using Blue Light.
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    Chapter 6 Photo Control of Protein Function Using Photoactive Yellow Protein
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    Chapter 7 Optogenetics
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    Chapter 8 Optogenetic Control of Pancreatic Islets
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    Chapter 9 Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
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    Chapter 10 Optogenetics
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    Chapter 11 Optogenetics
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    Chapter 12 Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches
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    Chapter 13 Optogenetic Modulation of Locomotor Activity on Free-Behaving Rats
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    Chapter 14 Combined Optogenetic and Chemogenetic Control of Neurons
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    Chapter 15 Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex
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    Chapter 16 An Optimized Calcium-Phosphate Transfection Method for Characterizing Genetically Encoded Tools in Primary Neurons
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    Chapter 17 Optogenetic Approaches for Mesoscopic Brain Mapping
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    Chapter 18 Optogenetic Tools for Confined Stimulation in Deep Brain Structures
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    Chapter 19 Optogenetics
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    Chapter 20 Optogenetic Light Crafting Tools for the Control of Cardiac Arrhythmias
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    Chapter 21 Inscribing Optical Excitability to Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary Cardiac Fibroblasts
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    Chapter 22 Optogenetic Engineering of Atrial Cardiomyocytes
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    Chapter 23 A Multichannel Recording System with Optical Stimulation for Closed-Loop Optogenetic Experiments
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    Chapter 24 Optogenetic Control of Fibroblast Growth Factor Receptor Signaling
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    Chapter 25 Protein Inactivation by Optogenetic Trapping in Living Cells
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    Chapter 26 Optogenetics
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    Chapter 27 Guidelines for Photoreceptor Engineering
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    Chapter 28 Erratum to: Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
Attention for Chapter 8: Optogenetic Control of Pancreatic Islets
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Chapter title
Optogenetic Control of Pancreatic Islets
Chapter number 8
Book title
Optogenetics
Published in
Methods in molecular biology, March 2016
DOI 10.1007/978-1-4939-3512-3_8
Pubmed ID
Book ISBNs
978-1-4939-3510-9, 978-1-4939-3512-3
Authors

Thomas M. Reinbothe, Inês G. Mollet

Editors

Arash Kianianmomeni

Abstract

In light of the emerging diabetes epidemic, new experimental approaches in islet research are needed to elucidate the mechanisms behind pancreatic islet dysfunction and to facilitate the development of more effective therapies. Optogenetics has created numerous new experimental tools enabling us to gain insights into processes little was known about before. The spatial and temporal precision that it can achieve is also attractive for studying the cells of the pancreatic islet and we set out to explore the possibilities of this technology for our purposes. We here describe how to use the islets of an "optogenetic beta-cell" mouse line in islet batch incubations and Ca(2+) imaging experiments. This protocol enables light-induced insulin release and provides an all-optical solution to control and measure intracellular Ca(2+) levels in pancreatic beta-cells. The technique is easy to set up and provides a useful tool for controlling the activity of distinct islet cell populations.

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 43%
Student > Ph. D. Student 1 14%
Student > Bachelor 1 14%
Student > Doctoral Student 1 14%
Professor > Associate Professor 1 14%
Other 0 0%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 57%
Pharmacology, Toxicology and Pharmaceutical Science 2 29%
Neuroscience 1 14%