↓ Skip to main content

Optogenetics

Overview of attention for book
Cover of 'Optogenetics'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Optogenetics
  3. Altmetric Badge
    Chapter 2 Optogenetics
  4. Altmetric Badge
    Chapter 3 Optogenetics
  5. Altmetric Badge
    Chapter 4 Reversible Photoregulation of Gene Expression and Translation
  6. Altmetric Badge
    Chapter 5 Controlling Protein Activity and Degradation Using Blue Light.
  7. Altmetric Badge
    Chapter 6 Photo Control of Protein Function Using Photoactive Yellow Protein
  8. Altmetric Badge
    Chapter 7 Optogenetics
  9. Altmetric Badge
    Chapter 8 Optogenetic Control of Pancreatic Islets
  10. Altmetric Badge
    Chapter 9 Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
  11. Altmetric Badge
    Chapter 10 Optogenetics
  12. Altmetric Badge
    Chapter 11 Optogenetics
  13. Altmetric Badge
    Chapter 12 Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches
  14. Altmetric Badge
    Chapter 13 Optogenetic Modulation of Locomotor Activity on Free-Behaving Rats
  15. Altmetric Badge
    Chapter 14 Combined Optogenetic and Chemogenetic Control of Neurons
  16. Altmetric Badge
    Chapter 15 Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex
  17. Altmetric Badge
    Chapter 16 An Optimized Calcium-Phosphate Transfection Method for Characterizing Genetically Encoded Tools in Primary Neurons
  18. Altmetric Badge
    Chapter 17 Optogenetic Approaches for Mesoscopic Brain Mapping
  19. Altmetric Badge
    Chapter 18 Optogenetic Tools for Confined Stimulation in Deep Brain Structures
  20. Altmetric Badge
    Chapter 19 Optogenetics
  21. Altmetric Badge
    Chapter 20 Optogenetic Light Crafting Tools for the Control of Cardiac Arrhythmias
  22. Altmetric Badge
    Chapter 21 Inscribing Optical Excitability to Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary Cardiac Fibroblasts
  23. Altmetric Badge
    Chapter 22 Optogenetic Engineering of Atrial Cardiomyocytes
  24. Altmetric Badge
    Chapter 23 A Multichannel Recording System with Optical Stimulation for Closed-Loop Optogenetic Experiments
  25. Altmetric Badge
    Chapter 24 Optogenetic Control of Fibroblast Growth Factor Receptor Signaling
  26. Altmetric Badge
    Chapter 25 Protein Inactivation by Optogenetic Trapping in Living Cells
  27. Altmetric Badge
    Chapter 26 Optogenetics
  28. Altmetric Badge
    Chapter 27 Guidelines for Photoreceptor Engineering
  29. Altmetric Badge
    Chapter 28 Erratum to: Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
Attention for Chapter 7: Optogenetics
Altmetric Badge

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
11 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Optogenetics
Chapter number 7
Book title
Optogenetics
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3512-3_7
Pubmed ID
Book ISBNs
978-1-4939-3510-9, 978-1-4939-3512-3
Authors

Schumacher, Charlotte Helene, Körschen, Heinz G, Nicol, Christopher, Gasser, Carlos, Seifert, Reinhard, Schwärzel, Martin, Möglich, Andreas, Charlotte Helene Schumacher, Heinz G. Körschen, Christopher Nicol, Carlos Gasser, Reinhard Seifert, Martin Schwärzel, Andreas Möglich

Editors

Arash Kianianmomeni

Abstract

As a transformative approach in neuroscience and cell biology, optogenetics grants control over manifold cellular events with unprecedented spatiotemporal definition, reversibility, and noninvasiveness. Sensory photoreceptors serve as genetically encoded, light-regulated actuators and hence embody the cornerstone of optogenetics. To expand the scope of optogenetics, ever more naturally occurring photoreceptors are being characterized, and synthetic photoreceptors with customized, light-regulated function are being engineered. Perturbational control over intracellular cyclic-nucleotide-monophosphate (cNMP) levels is achieved via sensory photoreceptors that catalyze the making and breaking of these second messengers in response to light. To facilitate discovery, engineering and quantitative characterization of such light-regulated cNMP actuators, we have developed an efficient fluorometric assay. Both the formation and the hydrolysis of cNMPs are accompanied by proton release which can be quantified with the fluorescent pH indicator 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF). This assay equally applies to nucleotide cyclases, e.g., blue-light-activated bPAC, and to cNMP phosphodiesterases, e.g., red-light-activated LAPD. Key benefits include potential for parallelization and automation, as well as suitability for both purified enzymes and crude cell lysates. The BCECF assay hence stands to accelerate discovery and characterization of light-regulated actuators of cNMP metabolism.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 27%
Researcher 3 27%
Student > Ph. D. Student 2 18%
Student > Doctoral Student 1 9%
Student > Postgraduate 1 9%
Other 0 0%
Unknown 1 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 45%
Medicine and Dentistry 2 18%
Pharmacology, Toxicology and Pharmaceutical Science 1 9%
Neuroscience 1 9%
Engineering 1 9%
Other 0 0%
Unknown 1 9%