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Optogenetics

Overview of attention for book
Cover of 'Optogenetics'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Optogenetics
  3. Altmetric Badge
    Chapter 2 Optogenetics
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    Chapter 3 Optogenetics
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    Chapter 4 Reversible Photoregulation of Gene Expression and Translation
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    Chapter 5 Controlling Protein Activity and Degradation Using Blue Light.
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    Chapter 6 Photo Control of Protein Function Using Photoactive Yellow Protein
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    Chapter 7 Optogenetics
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    Chapter 8 Optogenetic Control of Pancreatic Islets
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    Chapter 9 Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
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    Chapter 10 Optogenetics
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    Chapter 11 Optogenetics
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    Chapter 12 Optogenetic Control of Mammalian Ion Channels with Chemical Photoswitches
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    Chapter 13 Optogenetic Modulation of Locomotor Activity on Free-Behaving Rats
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    Chapter 14 Combined Optogenetic and Chemogenetic Control of Neurons
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    Chapter 15 Intracranial Injection of an Optogenetics Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in Rat Brain Cortex
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    Chapter 16 An Optimized Calcium-Phosphate Transfection Method for Characterizing Genetically Encoded Tools in Primary Neurons
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    Chapter 17 Optogenetic Approaches for Mesoscopic Brain Mapping
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    Chapter 18 Optogenetic Tools for Confined Stimulation in Deep Brain Structures
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    Chapter 19 Optogenetics
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    Chapter 20 Optogenetic Light Crafting Tools for the Control of Cardiac Arrhythmias
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    Chapter 21 Inscribing Optical Excitability to Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary Cardiac Fibroblasts
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    Chapter 22 Optogenetic Engineering of Atrial Cardiomyocytes
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    Chapter 23 A Multichannel Recording System with Optical Stimulation for Closed-Loop Optogenetic Experiments
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    Chapter 24 Optogenetic Control of Fibroblast Growth Factor Receptor Signaling
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    Chapter 25 Protein Inactivation by Optogenetic Trapping in Living Cells
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    Chapter 26 Optogenetics
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    Chapter 27 Guidelines for Photoreceptor Engineering
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    Chapter 28 Erratum to: Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
Attention for Chapter 5: Controlling Protein Activity and Degradation Using Blue Light.
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  • Above-average Attention Score compared to outputs of the same age and source (64th percentile)

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Citations

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Readers on

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49 Mendeley
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Chapter title
Controlling Protein Activity and Degradation Using Blue Light.
Chapter number 5
Book title
Optogenetics
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3512-3_5
Pubmed ID
Book ISBNs
978-1-4939-3510-9, 978-1-4939-3512-3
Authors

Anne P. Lutz, Christian Renicke, Christof Taxis

Editors

Arash Kianianmomeni

Abstract

Regulation of protein stability is a fundamental process in eukaryotic cells and pivotal to, e.g., cell cycle progression, faithful chromosome segregation, or protein quality control. Synthetic regulation of protein stability requires conditional degradation sequences (degrons) that induce a stability switch upon a specific signal. Fusion to a selected target protein permits to influence virtually every process in a cell. Light as signal is advantageous due to its precise applicability in time, space, quality, and quantity. Light control of protein stability was achieved by fusing the LOV2 photoreceptor domain of Arabidopsis thaliana phototropin1 with a synthetic degron (cODC1) derived from the carboxy-terminal degron of ornithine decarboxylase to obtain the photosensitive degron (psd) module. The psd module can be attached to the carboxy terminus of target proteins that are localized to the cytosol or nucleus to obtain light control over their stability. Blue light induces structural changes in the LOV2 domain, which in turn lead to activation of the degron and thus proteasomal degradation of the whole fusion protein. Variants of the psd module with diverse characteristics are useful to fine-tune the stability of a selected target at permissive (darkness) and restrictive conditions (blue light).

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 2%
Unknown 48 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 31%
Student > Master 10 20%
Researcher 9 18%
Student > Doctoral Student 4 8%
Student > Bachelor 4 8%
Other 6 12%
Unknown 1 2%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 18 37%
Agricultural and Biological Sciences 15 31%
Neuroscience 6 12%
Medicine and Dentistry 3 6%
Engineering 2 4%
Other 2 4%
Unknown 3 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 March 2016.
All research outputs
#9,025,332
of 14,376,801 outputs
Outputs from Methods in molecular biology
#3,000
of 8,743 outputs
Outputs of similar age
#144,371
of 265,548 outputs
Outputs of similar age from Methods in molecular biology
#20
of 88 outputs
Altmetric has tracked 14,376,801 research outputs across all sources so far. This one is in the 24th percentile – i.e., 24% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,743 research outputs from this source. They receive a mean Attention Score of 2.3. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,548 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 88 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.