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Mendeley readers
Attention Score in Context
| Title |
Protocol: transient expression system for functional genomics in the tropical tree Theobroma cacao L.
|
|---|---|
| Published in |
Plant Methods, March 2016
|
| DOI | 10.1186/s13007-016-0119-5 |
| Pubmed ID | |
| Authors |
Andrew S. Fister, Zi Shi, Yufan Zhang, Emily E. Helliwell, Siela N. Maximova, Mark J. Guiltinan |
| Abstract |
Theobroma cacao L., the source of cocoa, is a crop of significant economic value around the world. To facilitate the study of gene function in cacao we have developed a rapid Agrobacterium-mediated transient genetic transformation protocol. Here we present a detailed methodology for our transformation assay, as well as an assay for inoculation of cacao leaves with pathogens. Agrobacterium tumefaciens cultures are induced then vacuum-infiltrated into cacao leaves. Transformation success can be gauged 48 h after infiltration by observation of green fluorescent protein and by qRT-PCR. We clarify the characteristics of cacao leaf stages and demonstrate that our strategy efficiently transforms leaves of developmental stage C. The transformation protocol has high efficacy in stage C leaves of four of eight tested genotypes. We also present the functional analysis of cacao chitinase overexpression using the transient transformation system, which resulted in decreased pathogen biomass and lesion size after infection with Phytophthora tropicalis. Leaves expressing transgenes of interest can be used in subsequent functional genetic assays such as pathogen bioassay, metabolic analysis, gene expression analysis etc. This transformation protocol can be carried out in 1 day, and the transgenes expressing leaf tissue can be maintained in petri dishes for 5-7 days, allowing sufficient time for performance of additional downstream gene functional analysis. Application of these methods greatly increases the rapidity with which candidate genes with roles in defense can be tested. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 25% |
| United States | 1 | 25% |
| Spain | 1 | 25% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 92 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 2% |
| Unknown | 90 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 16% |
| Student > Ph. D. Student | 14 | 15% |
| Student > Bachelor | 13 | 14% |
| Student > Master | 7 | 8% |
| Student > Doctoral Student | 6 | 7% |
| Other | 16 | 17% |
| Unknown | 21 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 42 | 46% |
| Biochemistry, Genetics and Molecular Biology | 15 | 16% |
| Computer Science | 3 | 3% |
| Engineering | 2 | 2% |
| Environmental Science | 1 | 1% |
| Other | 6 | 7% |
| Unknown | 23 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 January 2018.
All research outputs
#12,656,356
of 22,856,968 outputs
Outputs from Plant Methods
#537
of 1,083 outputs
Outputs of similar age
#131,211
of 299,532 outputs
Outputs of similar age from Plant Methods
#7
of 15 outputs
Altmetric has tracked 22,856,968 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,083 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 299,532 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.