@WilliamPearman Check out this awesome paper: https://t.co/JHywDcWppq! After trying magnetic beads, columns, etc., it turns out an adapted CTAB/phenol-chloroform method works great for getting clean, HMW DNA from tricky crayfish exoskeleton.
@luke_earl @bsuther7 @IlianaIliana3 You can also "paste" the R1and R2 reads as in https://t.co/qKZFt9dBsR If you add an "N" in between, taxonomic assigment based on Wang for example shoud not be affected by the pasting.
@alpineedna The Zymo OneStep PCR Inhibitor Removal kit uses Zymo-Spin IV-HRC columns, which seem to contain Polyvinylpyrrolidon (https://t.co/6jHtn9wSFv) and maybe a silica matrix (https://t.co/9Gm0i18IYt). If thats still true, protocols described in https
RT @BarcodingPapers: Preparation of Amplicon Libraries for Metabarcoding of Marine Eukaryotes Using Illumina MiSeq: The Dual-PCR Method. ht…