↓ Skip to main content

Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR

Overview of attention for article published in Brazilian Journal of Microbiology, April 2016
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (65th percentile)
  • Good Attention Score compared to outputs of the same age and source (75th percentile)

Mentioned by

twitter
1 tweeter
wikipedia
1 Wikipedia page

Citations

dimensions_citation
16 Dimensions

Readers on

mendeley
33 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR
Published in
Brazilian Journal of Microbiology, April 2016
DOI 10.1016/j.bjm.2016.01.013
Pubmed ID
Authors

Liliana Rosa Gilardoni, Bárbara Fernández, Claudia Morsella, Laura Mendez, Ana María Jar, Fernando Alberto Paolicchi, Silvia Leonor Mundo

Abstract

The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900-PCR detection (IMS-IS1 PCR) was employed on milk samples from 265 lactating Holstein cows from Map infected and uninfected herds in Argentina. IMS-IS1 PCR results were analyzed and compared with those obtained from milk and fecal culture and serum ELISA. The extent of agreement between both tests was determined by the Kappa test. IMS-IS1 PCR showed a detection limit of 10(1) CFU of Map/mL of milk, when 50:50 mix of monoclonal and polyclonal antibodies were used to coat magnetic beads. All of the 118 samples from the Map uninfected herds were negative for the set of the tests. In Map infected herds, 80 out of 147 cows tested positive by milk IMS-IS1 PCR (55%), of which 2 (1.4%) were also positive by milk culture, 15 (10%) by fecal culture, and 20 (14%) by serum ELISA. Kappa statistics (95% CI) showed a slight agreement between the different tests (<0.20), and the proportions of agreement were ≤0.55. The IMS-IS1 PCR method detected Map in milk of the cows that were not positive in other techniques. This is the first report dealing with the application of IMS-IS1 PCR in the detection of Map in raw milk samples under field conditions in Argentina.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 3%
Unknown 32 97%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 21%
Student > Ph. D. Student 5 15%
Student > Bachelor 4 12%
Researcher 4 12%
Professor > Associate Professor 3 9%
Other 5 15%
Unknown 5 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 27%
Veterinary Science and Veterinary Medicine 6 18%
Immunology and Microbiology 5 15%
Biochemistry, Genetics and Molecular Biology 2 6%
Medicine and Dentistry 2 6%
Other 2 6%
Unknown 7 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 November 2020.
All research outputs
#6,094,281
of 19,430,502 outputs
Outputs from Brazilian Journal of Microbiology
#94
of 521 outputs
Outputs of similar age
#92,520
of 276,380 outputs
Outputs of similar age from Brazilian Journal of Microbiology
#5
of 20 outputs
Altmetric has tracked 19,430,502 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 521 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 276,380 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.