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Evaluation of an experimental rat model for comparative studies of bleaching agents

Overview of attention for article published in Journal of Applied Oral Science, February 2016
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Title
Evaluation of an experimental rat model for comparative studies of bleaching agents
Published in
Journal of Applied Oral Science, February 2016
DOI 10.1590/1678-775720150393
Pubmed ID
Authors

Cintra, Luciano Tavares Angelo, Benetti, Francine, Ferreira, Luciana Lousada, Rahal, Vanessa, Ervolino, Edilson, Jacinto, Rogério de Castilho, Gomes Filho, João Eduardo, Briso, André Luiz Fraga, Cintra, Luciano Tavares Angelo, Benetti, Francine, Ferreira, Luciana Lousada, Rahal, Vanessa, Ervolino, Edilson, Jacinto, Rogério de Castilho, Gomes Filho, João Eduardo, Briso, André Luiz Fraga

Abstract

Dental materials, in general, are tested in different animal models prior to their clinical use in humans, except for bleaching agents. Objectives To evaluate an experimental rat model for comparative studies of bleaching agents by investigating the influence of different concentrations and application times of H2O2 gel in the pulp tissue during in-office bleaching of rats' vital teeth. Material and methods The right and left maxillary molars of 50 Wistar rats were bleached with 20% and 35% H2O2 gels, respectively, for 5, 10, 15, 30, or 45 min (n=10 rats/group). Ten animals (control) were untreated. The rats were killed after 2 or 30 days, and the maxillae were examined by light microscopy. Inflammation was evaluated by histomorphometric analysis with inflammatory cell counting in the coronal and radicular thirds of the pulp. The counting of fibroblasts was also performed. Scores were attributed to the odontoblastic layer and to vascular changes. The tertiary dentin area and the pulp chamber central area were histomorphometrically measured. Data were compared by the analysis of variance and the Kruskal-Wallis test (p<0.05). Results After 2 days, the amount of inflammatory cells increased in the occlusal third of the coronal pulp until the time of 15 min for both concentrations of bleaching gels. In 30 and 45 min groups of each concentration, the number of inflammatory cells decreased along with the appearance of necrotic areas. After 30 days, a reduction in the pulp chamber central area and an enlargement of tertiary dentin area were observed without the detection of inflammation areas. Conclusion The rat model of extra coronal bleaching showed to be adequate for bleaching protocols studies, as it was possible to observe alterations in the pulp tissues and in the tooth structure caused by different concentrations and periods of application of bleaching agents.

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Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 2%
Germany 1 2%
Unknown 39 95%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 8 20%
Student > Master 6 15%
Professor 4 10%
Other 3 7%
Student > Doctoral Student 3 7%
Other 7 17%
Unknown 10 24%
Readers by discipline Count As %
Medicine and Dentistry 23 56%
Agricultural and Biological Sciences 1 2%
Chemistry 1 2%
Chemical Engineering 1 2%
Unknown 15 37%