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Evaluation of resistance acquisition during tuberculosis treatment using whole genome sequencing

Overview of attention for article published in The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases, March 2016
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Title
Evaluation of resistance acquisition during tuberculosis treatment using whole genome sequencing
Published in
The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases, March 2016
DOI 10.1016/j.bjid.2016.01.004
Pubmed ID
Authors

Silva Feliciano, Cinara, Rodrigues Plaça, Jessica, Peronni, Kamila, Araújo Silva, Wilson, Roberto Bollela, Valdes

Abstract

Tuberculosis (TB) is still considered a major global public health problem in the world and there is a concern about the worldwide increase of drug-resistance (DR). This paper describes the analysis of three Mycobacterium tuberculosis isolates from a single patient collected over a long treatment period of time. DR was initially investigated through phenotypic testing, followed by line probe assays (LPAs) and whole genome sequencing (WGS). It presents an intriguing situation where a multidrug-resistant (MDR-) TB case was diagnosed and treated based only on late phenotypic drug susceptibility testing of isolate 1. During the treatment, another two isolates were cultivated: isolate 2, nine months after starting MDR-TB treatment; and isolate 3, cultivated five months later, during regular use of anti-TB drugs. These two isolates were evaluated using molecular LPA and WGS, retrospectively. All mutations detected by LPA were also detected in the WGS, including conversion from fluoroquinolones susceptibility to resistance from isolate 2 to isolate 3. WGS showed additional mutations, including some which may confer resistance to other drugs not tested (terizidone/cycloserine) and mutations with no correspondent resistance in drug susceptibility testing (streptomycin and second-line injectable drugs).

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Mendeley readers

The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 2%
Unknown 57 98%

Demographic breakdown

Readers by professional status Count As %
Student > Master 14 24%
Student > Bachelor 8 14%
Researcher 6 10%
Student > Ph. D. Student 6 10%
Other 4 7%
Other 10 17%
Unknown 10 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 22%
Medicine and Dentistry 10 17%
Agricultural and Biological Sciences 7 12%
Immunology and Microbiology 7 12%
Computer Science 3 5%
Other 5 9%
Unknown 13 22%