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Biological roles of filamin a in prostate cancer cells

Overview of attention for article published in International braz j urol, September 2019
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Title
Biological roles of filamin a in prostate cancer cells
Published in
International braz j urol, September 2019
DOI 10.1590/s1677-5538.ibju.2018.0535
Pubmed ID
Authors

Xue-Chao Li, Chuan-Xi Huang, Shi-Kui Wu, Lan Yu, Guang-Jian Zhou, Li-Jun Chen

Abstract

This study aims to investigate the association of filamin A with the function and morphology of prostate cancer (PCa) cells, and explore the role of filamin A in the development of PCa, in order to analyze its significance in the evolvement of PCa. A stably transfected cell line, in which filamin A expression was suppressed by RNA interference, was first established. Then, the effects of the sup¬pression of filamin A gene expression on the biological characteristics of human PCa LNCaP cells were observed through cell morphology, in vitro cell growth curve, soft agar cloning assay, and scratch test. A cell line model with a low expression of filamin A was successfully con¬structed on the basis of LNCaP cells. The morphology of cells transfected with plasmid pSilencer-filamin A was the following: Cells were loosely arranged, had less connec¬tion with each other, had fewer tentacles, and presented a fibrous look. The growth rate of LNCap cells was faster than cells transfected with plasmid pSilencer-filamin A (P<0.05). The clones of LNCap cells in the soft agar cloning assay was significantly fewer than that of cells stably transfected with plasmid pSilencer-filamin A (P<0.05). Cells stably transfected with plasmid pSilencer-filamin A presented with a stronger healing and migration ability compared to LNCap cells (healing rate was 32.2% and 12.1%, respectively; P<0.05). The expression of the filamin A gene inhibited the malignant development of LNCap cells. Therefore, the filamin A gene may be a tumor suppressor gene.

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Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 22%
Student > Bachelor 2 22%
Unspecified 1 11%
Student > Doctoral Student 1 11%
Student > Master 1 11%
Other 1 11%
Unknown 1 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 33%
Unspecified 1 11%
Medicine and Dentistry 1 11%
Unknown 4 44%