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Design and synthesis of a minimal bacterial genome

Overview of attention for article published in Science, March 2016
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#10 of 39,900)
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Readers on

mendeley
1275 Mendeley
citeulike
8 CiteULike
Title
Design and synthesis of a minimal bacterial genome
Published in
Science, March 2016
DOI 10.1126/science.aad6253
Pubmed ID
Authors

Clyde A. Hutchison, Ray-Yuan Chuang, Vladimir N. Noskov, Nacyra Assad-Garcia, Thomas J. Deerinck, Mark H. Ellisman, John Gill, Krishna Kannan, Bogumil J. Karas, Li Ma, James F. Pelletier, Zhi-Qing Qi, R. Alexander Richter, Elizabeth A. Strychalski, Lijie Sun, Yo Suzuki, Billyana Tsvetanova, Kim S. Wise, Hamilton O. Smith, John I. Glass, Chuck Merryman, Daniel G. Gibson, J. Craig Venter, Hutchison, Clyde A, Chuang, Ray-Yuan, Noskov, Vladimir N, Assad-Garcia, Nacyra, Deerinck, Thomas J, Ellisman, Mark H, Gill, John, Kannan, Krishna, Karas, Bogumil J, Ma, Li, Pelletier, James F, Qi, Zhi-Qing, Richter, R Alexander, Strychalski, Elizabeth A, Sun, Lijie, Suzuki, Yo, Tsvetanova, Billyana, Wise, Kim S, Smith, Hamilton O, Glass, John I, Merryman, Chuck, Gibson, Daniel G, Venter, J Craig

Abstract

We used whole-genome design and complete chemical synthesis to minimize the 1079-kilobase pair synthetic genome of Mycoplasma mycoides JCVI-syn1.0. An initial design, based on collective knowledge of molecular biology combined with limited transposon mutagenesis data, failed to produce a viable cell. Improved transposon mutagenesis methods revealed a class of quasi-essential genes that are needed for robust growth, explaining the failure of our initial design. Three cycles of design, synthesis, and testing, with retention of quasi-essential genes, produced JCVI-syn3.0 (531 kilobase pairs, 473 genes), which has a genome smaller than that of any autonomously replicating cell found in nature. JCVI-syn3.0 retains almost all genes involved in the synthesis and processing of macromolecules. Unexpectedly, it also contains 149 genes with unknown biological functions. JCVI-syn3.0 is a versatile platform for investigating the core functions of life and for exploring whole-genome design.

Twitter Demographics

The data shown below were collected from the profiles of 1,256 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 1,275 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 38 3%
Germany 14 1%
United Kingdom 14 1%
Japan 8 <1%
Spain 7 <1%
Switzerland 7 <1%
France 6 <1%
Canada 5 <1%
Italy 4 <1%
Other 46 4%
Unknown 1126 88%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 363 28%
Researcher 241 19%
Student > Bachelor 220 17%
Student > Master 187 15%
Student > Doctoral Student 57 4%
Other 207 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 652 51%
Biochemistry, Genetics and Molecular Biology 265 21%
Chemistry 72 6%
Medicine and Dentistry 55 4%
Engineering 47 4%
Other 184 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 2427. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 May 2017.
All research outputs
#146
of 7,749,358 outputs
Outputs from Science
#10
of 39,900 outputs
Outputs of similar age
#16
of 278,401 outputs
Outputs of similar age from Science
#1
of 1,173 outputs
Altmetric has tracked 7,749,358 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 39,900 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 31.8. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 278,401 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 1,173 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.