Design and synthesis of a minimal bacterial genome

Overview of attention for article published in Science, March 2016
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#9 of 39,082)
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Readers on

mendeley
1251 Mendeley
citeulike
8 CiteULike
Title
Design and synthesis of a minimal bacterial genome
Published in
Science, March 2016
DOI 10.1126/science.aad6253
Pubmed ID
Authors

Clyde A. Hutchison, Ray-Yuan Chuang, Vladimir N. Noskov, Nacyra Assad-Garcia, Thomas J. Deerinck, Mark H. Ellisman, John Gill, Krishna Kannan, Bogumil J. Karas, Li Ma, James F. Pelletier, Zhi-Qing Qi, R. Alexander Richter, Elizabeth A. Strychalski, Lijie Sun, Yo Suzuki, Billyana Tsvetanova, Kim S. Wise, Hamilton O. Smith, John I. Glass, Chuck Merryman, Daniel G. Gibson, J. Craig Venter, Hutchison, Clyde A, Chuang, Ray-Yuan, Noskov, Vladimir N, Assad-Garcia, Nacyra, Deerinck, Thomas J, Ellisman, Mark H, Gill, John, Kannan, Krishna, Karas, Bogumil J, Ma, Li, Pelletier, James F, Qi, Zhi-Qing, Richter, R Alexander, Strychalski, Elizabeth A, Sun, Lijie, Suzuki, Yo, Tsvetanova, Billyana, Wise, Kim S, Smith, Hamilton O, Glass, John I, Merryman, Chuck, Gibson, Daniel G, Venter, J Craig

Abstract

We used whole-genome design and complete chemical synthesis to minimize the 1079-kilobase pair synthetic genome of Mycoplasma mycoides JCVI-syn1.0. An initial design, based on collective knowledge of molecular biology combined with limited transposon mutagenesis data, failed to produce a viable cell. Improved transposon mutagenesis methods revealed a class of quasi-essential genes that are needed for robust growth, explaining the failure of our initial design. Three cycles of design, synthesis, and testing, with retention of quasi-essential genes, produced JCVI-syn3.0 (531 kilobase pairs, 473 genes), which has a genome smaller than that of any autonomously replicating cell found in nature. JCVI-syn3.0 retains almost all genes involved in the synthesis and processing of macromolecules. Unexpectedly, it also contains 149 genes with unknown biological functions. JCVI-syn3.0 is a versatile platform for investigating the core functions of life and for exploring whole-genome design.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 1,251 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 44 4%
United Kingdom 17 1%
Germany 14 1%
Japan 9 <1%
France 8 <1%
Canada 7 <1%
Spain 7 <1%
Switzerland 7 <1%
Italy 5 <1%
Other 53 4%
Unknown 1080 86%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 369 29%
Researcher 238 19%
Student > Bachelor 209 17%
Student > Master 185 15%
Professor > Associate Professor 55 4%
Other 195 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 646 52%
Biochemistry, Genetics and Molecular Biology 266 21%
Chemistry 67 5%
Medicine and Dentistry 58 5%
Engineering 47 4%
Other 167 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 2428. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 March 2017.
All research outputs
#130
of 7,424,620 outputs
Outputs from Science
#9
of 39,082 outputs
Outputs of similar age
#14
of 275,230 outputs
Outputs of similar age from Science
#1
of 1,173 outputs
Altmetric has tracked 7,424,620 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 39,082 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 30.7. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 275,230 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 1,173 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.