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Oligonucleotide-Based Therapies

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Cover of 'Oligonucleotide-Based Therapies'

Table of Contents

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    Book Overview
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    Chapter 1 Chemical Development of Therapeutic Oligonucleotides
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    Chapter 2 Tissue-Specific Delivery of Oligonucleotides
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    Chapter 3 Designing siRNA and Evaluating Its Effect on RNA Targets Using qPCR and Western Blot
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    Chapter 4 Antisense Oligonucleotides for Splice Modulation: Assessing Splice Switching Efficacy
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    Chapter 5 Assessing Oligonucleotide Binding to Double-Stranded DNA
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    Chapter 6 Modeling and Simulation of Oligonucleotide Hybrids: Outlining a Strategy
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    Chapter 7 Characterization of Peptide–Oligonucleotide Complexes Using Electron Microscopy, Dynamic Light Scattering, and Protease Resistance Assay
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    Chapter 8 Click-Shielded and Targeted Lipopolyplexes
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    Chapter 9 Attachment of Peptides to Oligonucleotides on Solid Support Using Copper(I)-Catalyzed Huisgen 1,3-Dipolar Cycloaddition
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    Chapter 10 Characterizing Oligonucleotide Uptake in Cultured Cells: A Case Study Using AS1411 Aptamer
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    Chapter 11 Measuring the Action of Oligonucleotide Therapeutics in the Lung at the Cell Type-Specific Level by Tissue Disruption and Cell Sorting (TDCS)
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    Chapter 12 Delivery of Antisense Oligonucleotides Mediated by a Hydrogel System: In Vitro and In Vivo Application in the Context of Spinal Cord Injury
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    Chapter 13 Evaluation of Cell-Penetrating Peptide Delivery of Antisense Oligonucleotides for Therapeutic Efficacy in Spinal Muscular Atrophy
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    Chapter 14 Injection and Infusion of Compounds to the Central Nervous System
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    Chapter 15 In Vitro Assessment of the Hepatotoxicity Potential of Therapeutic Oligonucleotides
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    Chapter 16 Identifying Suitable Target Regions and Analyzing Off-Target Effects of Therapeutic Oligonucleotides
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    Chapter 17 RACE-SEQ and Population-Wide Polymorphism Susceptibility Testing for Endonucleolytically Active, RNA-Targeting Therapeutics
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    Chapter 18 Studying the Biotransformation of Phosphorothioate-Containing Oligonucleotide Drugs by LC-MS
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    Chapter 19 ADME: Assessing Pharmacokinetic–Pharmacodynamic Parameters of Oligonucleotides
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