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Visualizing the replicating HSV-1 virus using STED super-resolution microscopy

Overview of attention for article published in Virology Journal, April 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

Mentioned by

news
1 news outlet
blogs
1 blog
twitter
6 X users
facebook
1 Facebook page
googleplus
1 Google+ user

Citations

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10 Dimensions

Readers on

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40 Mendeley
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Title
Visualizing the replicating HSV-1 virus using STED super-resolution microscopy
Published in
Virology Journal, April 2016
DOI 10.1186/s12985-016-0521-7
Pubmed ID
Authors

Zhuoran Li, Ce Fang, Yuanyuan Su, Hongmei Liu, Fengchao Lang, Xin Li, Guijun Chen, Danfeng Lu, Jumin Zhou

Abstract

Replication of viral genome is the central event during the lytic infectious cycle of herpes simplex virus 1 (HSV-1). However, the details of HSV-1 replication process are still elusive due to the limitations of current molecular and conventional fluorescent microscopy methods. Stimulated emission depletion (STED) microscopy is one of the recently available super-resolution techniques allowing observation at sub-diffraction resolution. To gain new insight into HSV-1 replication, we used a combination of stimulated emission depletion microscopy, fluorescence in situ hybridization (FISH) and immunofluorescence (IF) to observe the HSV-1 replication process. Using two colored probes labeling the same region of HSV-1 genome, the two probes highly correlated in both pre-replication and replicating genomes. In comparison, when probes from different regions were used, the average distance between the two probes increased after the virus enters replication, suggesting that the HSV-1 genome undergoes dynamic structure changes from a compact to a relaxed formation and occupies larger space as it enters replication. Using FISH and IF, viral single strand binding protein ICP8 was seen closely positioned with HSV-1 genome. In contrast, ICP8 and host RNA polymerase II were less related. This result suggests that ICP8 marked regions of DNA replication are spatially separated from regions of active transcription, represented by the elongating form of RNA polymerase II within the viral replication compartments. Comparing HSV-1 genomes at early stage of replication with that in later stage, we also noted overall increases among different values. These results suggest stimulated emission depletion microscopy is capable of investigating events during HSV-1 replication. 1) Replicating HSV-1 genome could be observed by super-resolution microscopy; 2) Viral genome expands spatially during replication; 3) Viral replication and transcription are partitioned into different sub-structures within the replication compartments.

X Demographics

X Demographics

The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 3%
Unknown 39 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 28%
Student > Bachelor 5 13%
Professor 4 10%
Student > Master 3 8%
Researcher 3 8%
Other 6 15%
Unknown 8 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 35%
Agricultural and Biological Sciences 9 23%
Immunology and Microbiology 3 8%
Physics and Astronomy 2 5%
Medicine and Dentistry 1 3%
Other 2 5%
Unknown 9 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 April 2016.
All research outputs
#1,700,357
of 22,860,626 outputs
Outputs from Virology Journal
#122
of 3,051 outputs
Outputs of similar age
#30,329
of 300,860 outputs
Outputs of similar age from Virology Journal
#3
of 52 outputs
Altmetric has tracked 22,860,626 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,051 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.7. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 300,860 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.