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Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli

Overview of attention for article published in Microbial Cell Factories, April 2016
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (80th percentile)
  • High Attention Score compared to outputs of the same age and source (86th percentile)

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2 X users
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2 patents
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2 Facebook pages

Citations

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51 Mendeley
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Title
Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli
Published in
Microbial Cell Factories, April 2016
DOI 10.1186/s12934-016-0457-z
Pubmed ID
Authors

Fabián Rueda, María Virtudes Céspedes, Alejandro Sánchez-Chardi, Joaquin Seras-Franzoso, Mireia Pesarrodona, Neus Ferrer-Miralles, Esther Vázquez, Ursula Rinas, Ugutz Unzueta, Uwe Mamat, Ramón Mangues, Elena García-Fruitós, Antonio Villaverde

Abstract

Production of recombinant drugs in process-friendly endotoxin-free bacterial factories targets to a lessened complexity of the purification process combined with minimized biological hazards during product application. The development of nanostructured recombinant materials in innovative nanomedical activities expands such a need beyond plain functional polypeptides to complex protein assemblies. While Escherichia coli has been recently modified for the production of endotoxin-free proteins, no data has been so far recorded regarding how the system performs in the fabrication of smart nanostructured materials. We have here explored the nanoarchitecture and in vitro and in vivo functionalities of CXCR4-targeted, self-assembling protein nanoparticles intended for intracellular delivery of drugs and imaging agents in colorectal cancer. Interestingly, endotoxin-free materials exhibit a distinguishable architecture and altered size and target cell penetrability than counterparts produced in conventional E. coli strains. These variant nanoparticles show an eventual proper biodistribution and highly specific and exclusive accumulation in tumor upon administration in colorectal cancer mice models, indicating a convenient display and function of the tumor homing peptides and high particle stability under physiological conditions. The observations made here support the emerging endotoxin-free E. coli system as a robust protein material producer but are also indicative of a particular conformational status and organization of either building blocks or oligomers. This appears to be promoted by multifactorial stress-inducing conditions upon engineering of the E. coli cell envelope, which impacts on the protein quality control of the cell factory.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 51 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 20%
Student > Bachelor 6 12%
Student > Ph. D. Student 5 10%
Other 4 8%
Student > Postgraduate 4 8%
Other 12 24%
Unknown 10 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 17 33%
Agricultural and Biological Sciences 6 12%
Unspecified 3 6%
Medicine and Dentistry 2 4%
Social Sciences 2 4%
Other 6 12%
Unknown 15 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 October 2020.
All research outputs
#3,636,398
of 22,860,626 outputs
Outputs from Microbial Cell Factories
#173
of 1,603 outputs
Outputs of similar age
#58,282
of 300,819 outputs
Outputs of similar age from Microbial Cell Factories
#4
of 37 outputs
Altmetric has tracked 22,860,626 research outputs across all sources so far. Compared to these this one has done well and is in the 84th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,603 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done well, scoring higher than 89% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 300,819 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 37 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.