↓ Skip to main content

Growth-factor reduced Matrigel source influences stem cell derived brain microvascular endothelial cell barrier properties

Overview of attention for article published in Fluids and Barriers of the CNS, April 2016
Altmetric Badge

About this Attention Score

  • Among the highest-scoring outputs from this source (#41 of 102)
  • Average Attention Score compared to outputs of the same age

Mentioned by

twitter
2 tweeters

Citations

dimensions_citation
20 Dimensions

Readers on

mendeley
36 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Growth-factor reduced Matrigel source influences stem cell derived brain microvascular endothelial cell barrier properties
Published in
Fluids and Barriers of the CNS, April 2016
DOI 10.1186/s12987-016-0030-5
Pubmed ID
Authors

Ronak Patel, Abraham J. Alahmad

Abstract

Patient-derived induced pluripotent stem cells (iPSCs) are an innovative source as an in vitro model for neurological diseases. Recent studies have demonstrated the differentiation of brain microvascular endothelial cells (BMECs) from various stem cell sources, including iPSC lines. However, the impact of the culturing conditions used to maintain such stem cell pluripotency on their ability to differentiate into BMECs remains undocumented. In this study, we investigated the effect of different sources of Matrigel and stem cell maintenance medium on BMEC differentiation efficiency. The IMR90-c4 iPSC line was maintained on mTeSR1 or in essential-8 (E-8) medium on growth factor-reduced (GFR) Matrigel from three different manufacturers. Cells were differentiated into BMECs following published protocols. The phenotype of BMEC monolayers was assessed by immunocytochemistry. Barrier function was assessed by transendothelial electrical resistance (TEER) and permeability to sodium fluorescein, whereas the presence of drug efflux pumps was assessed by uptake assay using fluorescent substrates. Stem cell maintenance medium had little effect on the yield and barrier phenotype of IMR90-derived BMECs. The source of GFR-Matrigel used for the differentiation process significantly impacted the ability of IMR90-derived BMECs to form tight monolayers, as measured by TEER and fluorescein permeability. However, the Matrigel source had minimal effect on BMEC phenotype and drug efflux pump activity. This study supports the ability to differentiate BMECs from iPSCs grown in mTeSR1 or E-8 medium and also suggests that the origin of GFR-Matrigel has a marked inpact on BMEC barrier properties.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 36 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 39%
Student > Bachelor 7 19%
Researcher 4 11%
Student > Master 4 11%
Other 2 6%
Other 1 3%
Unknown 4 11%
Readers by discipline Count As %
Medicine and Dentistry 7 19%
Biochemistry, Genetics and Molecular Biology 7 19%
Neuroscience 5 14%
Agricultural and Biological Sciences 4 11%
Engineering 3 8%
Other 4 11%
Unknown 6 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 April 2016.
All research outputs
#3,682,771
of 7,551,260 outputs
Outputs from Fluids and Barriers of the CNS
#41
of 102 outputs
Outputs of similar age
#135,330
of 269,823 outputs
Outputs of similar age from Fluids and Barriers of the CNS
#2
of 3 outputs
Altmetric has tracked 7,551,260 research outputs across all sources so far. This one is in the 48th percentile – i.e., 48% of other outputs scored the same or lower than it.
So far Altmetric has tracked 102 research outputs from this source. They receive a mean Attention Score of 2.8. This one has gotten more attention than average, scoring higher than 54% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 269,823 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one.