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Horizontal Gene Transfer

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Cover of 'Horizontal Gene Transfer'

Table of Contents

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    Book Overview
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    Chapter 1 Plasmid DNA Isolation and Visualization: Isolation and Characterization of Plasmids from Clinical Samples
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    Chapter 2 Isolation and Visualization of Plasmids from Gram-Positive Bacteria of Interest in Public Health
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    Chapter 3 Detection, Isolation, and Characterization of Plasmids in the Environment
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    Chapter 4 Bacteriophage Isolation and Characterization: Phages of Escherichia coli
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    Chapter 5 Detection and Characterization of Transposons in Bacteria
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    Chapter 6 Measuring Plasmid Conjugation Using Antibiotic Selection
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    Chapter 7 Measuring Plasmid Conjugation Using Fluorescent Reporters
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    Chapter 8 Methods to Quantify DNA Transfer in Enterococcus
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    Chapter 9 Quantifying and Characterizing Distributive Conjugal Transfer in Mycobacterium smegmatis
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    Chapter 10 Spectrophotometric Assays to Quantify the Activity of T4SS ATPases
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    Chapter 11 Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis
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    Chapter 12 First Biochemical Steps on Bacterial Transposition Pathways
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    Chapter 13 Natural Transformation in Escherichia coli
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    Chapter 14 Integron Identification in Bacterial Genomes and Cassette Recombination Assays
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    Chapter 15 Methods to Identify and Analyze Vesicle-Protected DNA Transfer
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    Chapter 16 Measuring Plasmid Stability in Gram-Negative Bacteria
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    Chapter 17 Methods for the Analysis and Characterization of Defense Mechanisms Against Horizontal Gene Transfer: CRISPR Systems
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    Chapter 18 The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements
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    Chapter 19 Identifying Conjugative Plasmids and Integrative Conjugative Elements with CONJscan
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    Chapter 20 PlasmidFinder and In Silico pMLST: Identification and Typing of Plasmid Replicons in Whole-Genome Sequencing (WGS)
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    Chapter 21 MOBscan: Automated Annotation of MOB Relaxases
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    Chapter 22 Plasmid Typing and Classification
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    Chapter 23 Plasmid Reconstruction from Next-Gen Data: A Detailed Protocol for the Use of PLACNETw for the Reconstruction of Plasmids from WGS Datasets
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    Chapter 24 Statistical Analysis of Accessory Genome
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    Chapter 25 Inferring Horizontal Gene Transfer with DarkHorse, Phylomizer, and ETE Toolkits
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    Chapter 26 Methods to Study Fitness and Compensatory Adaptation in Plasmid-Carrying Bacteria
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    Chapter 27 A Broad Host Range Plasmid-Based Roadmap for ssDNA-Based Recombineering in Gram-Negative Bacteria
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    Chapter 28 Conjugative Assembly Genome Engineering (CAGE)
Attention for Chapter 12: First Biochemical Steps on Bacterial Transposition Pathways
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