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Induction of Human iPSC-Derived Cardiomyocyte Proliferation Revealed by Combinatorial Screening in High Density Microbioreactor Arrays

Overview of attention for article published in Scientific Reports, April 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • High Attention Score compared to outputs of the same age and source (86th percentile)

Mentioned by

news
1 news outlet
twitter
11 tweeters

Citations

dimensions_citation
48 Dimensions

Readers on

mendeley
114 Mendeley
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Title
Induction of Human iPSC-Derived Cardiomyocyte Proliferation Revealed by Combinatorial Screening in High Density Microbioreactor Arrays
Published in
Scientific Reports, April 2016
DOI 10.1038/srep24637
Pubmed ID
Authors

Drew M. Titmarsh, Nick R. Glass, Richard J. Mills, Alejandro Hidalgo, Ernst J. Wolvetang, Enzo R. Porrello, James E. Hudson, Justin J. Cooper-White

Abstract

Inducing cardiomyocyte proliferation in post-mitotic adult heart tissue is attracting significant attention as a therapeutic strategy to regenerate the heart after injury. Model animal screens have identified several candidate signalling pathways, however, it remains unclear as to what extent these pathways can be exploited, either individually or in combination, in the human system. The advent of human cardiac cells from directed differentiation of human pluripotent stem cells (hPSCs) now provides the ability to interrogate human cardiac biology in vitro, but it remains difficult with existing culture formats to simply and rapidly elucidate signalling pathway penetrance and interplay. To facilitate high-throughput combinatorial screening of candidate biologicals or factors driving relevant molecular pathways, we developed a high-density microbioreactor array (HDMA) - a microfluidic cell culture array containing 8100 culture chambers. We used HDMAs to combinatorially screen Wnt, Hedgehog, IGF and FGF pathway agonists. The Wnt activator CHIR99021 was identified as the most potent molecular inducer of human cardiomyocyte proliferation, inducing cell cycle activity marked by Ki67, and an increase in cardiomyocyte numbers compared to controls. The combination of human cardiomyocytes with the HDMA provides a versatile and rapid tool for stratifying combinations of factors for heart regeneration.

Twitter Demographics

The data shown below were collected from the profiles of 11 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 114 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 <1%
United States 1 <1%
Russia 1 <1%
Singapore 1 <1%
Unknown 110 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 31 27%
Researcher 24 21%
Student > Master 16 14%
Student > Bachelor 11 10%
Student > Doctoral Student 6 5%
Other 15 13%
Unknown 11 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 37 32%
Agricultural and Biological Sciences 24 21%
Engineering 17 15%
Medicine and Dentistry 12 11%
Pharmacology, Toxicology and Pharmaceutical Science 3 3%
Other 6 5%
Unknown 15 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 18. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 January 2020.
All research outputs
#1,639,228
of 21,749,011 outputs
Outputs from Scientific Reports
#15,127
of 116,258 outputs
Outputs of similar age
#28,674
of 279,633 outputs
Outputs of similar age from Scientific Reports
#439
of 3,197 outputs
Altmetric has tracked 21,749,011 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 116,258 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 17.8. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,633 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 3,197 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.