Chapter title |
Development of Rabies Virus-Like Particles for Vaccine Applications: Production, Characterization, and Protection Studies.
|
---|---|
Chapter number | 7 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3387-7_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3385-3, 978-1-4939-3387-7
|
Authors |
Diego Fontana, Marina Etcheverrigaray, Ricardo Kratje, Claudio Prieto |
Editors |
Sunil Thomas |
Abstract |
Rabies is a viral infection of the central nervous system for which vaccination is the only treatment possible. Besides preexposure, vaccination is highly recommended for people living in endemic areas, veterinarians, and laboratory workers. Our group has developed rabies virus-like particles (RV-VLPs) with immunogenic features expressed in mammalian cells for vaccine applications. In this chapter the methods to obtain and characterize a stable HEK293 cell line expressing RV-VLPs are detailed. Further, analytical ultracentrifugation steps to purify the obtained VLPs are developed, as well as western blot, dynamic light scattering, and immunogold electron microscopy to analyze the size, distribution, shape, and antigenic conformation of the purified particles. Finally, immunization protocols are described to study the immunogenicity of RV-VLPs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 25% |
Student > Master | 2 | 10% |
Student > Doctoral Student | 2 | 10% |
Student > Bachelor | 1 | 5% |
Professor | 1 | 5% |
Other | 5 | 25% |
Unknown | 4 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 25% |
Agricultural and Biological Sciences | 3 | 15% |
Veterinary Science and Veterinary Medicine | 2 | 10% |
Immunology and Microbiology | 2 | 10% |
Medicine and Dentistry | 1 | 5% |
Other | 1 | 5% |
Unknown | 6 | 30% |