Chapter title |
Application of Droplet Digital PCR to Validate Rift Valley Fever Vaccines.
|
---|---|
Chapter number | 10 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3387-7_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3385-3, 978-1-4939-3387-7
|
Authors |
Hoai J. Ly, Nandadeva Lokugamage, Tetsuro Ikegami |
Editors |
Sunil Thomas |
Abstract |
Droplet Digital™ polymerase chain reaction (ddPCR™) is a promising technique that quantitates the absolute concentration of nucleic acids in a given sample. This technique utilizes water-in-oil emulsion technology, a system developed by Bio-Rad Laboratories that partitions a single sample into thousands of nanoliter-sized droplets and counts nucleic acid molecules encapsulated in each individual particle as one PCR reaction. This chapter discusses the applications and methodologies of ddPCR for development of Rift Valley fever (RVF) vaccine, using an example that measures RNA copy numbers of a live-attenuated MP-12 vaccine from virus stocks, infected cells, or animal blood. We also discuss how ddPCR detects a reversion mutant of MP-12 from virus stocks accurately. The use of ddPCR improves the quality control of live-attenuated vaccines in the seed lot systems. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 4 | 24% |
Student > Postgraduate | 2 | 12% |
Professor > Associate Professor | 2 | 12% |
Student > Master | 1 | 6% |
Other | 1 | 6% |
Other | 1 | 6% |
Unknown | 6 | 35% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 3 | 18% |
Immunology and Microbiology | 2 | 12% |
Medicine and Dentistry | 2 | 12% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Computer Science | 1 | 6% |
Other | 3 | 18% |
Unknown | 5 | 29% |