Chapter title |
Designing Efficacious Vesicular Stomatitis Virus-Vectored Vaccines Against Ebola Virus.
|
---|---|
Chapter number | 12 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3387-7_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3385-3, 978-1-4939-3387-7
|
Authors |
Gary Wong Ph.D., Xiangguo Qiu M.D., Gary Wong, Xiangguo Qiu |
Editors |
Sunil Thomas |
Abstract |
Infection with the Ebola virus (EBOV) causes an aggressive hemorrhagic disease in humans and nonhuman primates. Traditional approaches, such as vaccination with inactivated virion preparations, have had limited efficacy, whereas immunization with live-attenuated EBOV is not feasible due to the highly lethal nature of the pathogen. This has necessitated the development of other approaches towards an effective EBOV vaccine. Over the past decade, recombinant viruses expressing the EBOV glycoprotein (GP) have constituted the most promising platforms, as evidenced by their ability to protect naïve nonhuman primates from a lethal EBOV challenge. The vesicular stomatitis virus (VSV) is one such vector and is currently progressing through the clinical pipeline. This chapter presents methodologies for the design, cloning, rescue, and preparation of live, recombinant VSV vaccines expressing GP for research purposes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 35 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 14% |
Other | 5 | 14% |
Student > Master | 5 | 14% |
Librarian | 4 | 11% |
Student > Bachelor | 4 | 11% |
Other | 6 | 17% |
Unknown | 6 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 20% |
Nursing and Health Professions | 5 | 14% |
Immunology and Microbiology | 4 | 11% |
Medicine and Dentistry | 4 | 11% |
Agricultural and Biological Sciences | 4 | 11% |
Other | 3 | 9% |
Unknown | 8 | 23% |