Chapter title |
Ricin-Holotoxin-Based Vaccines: Induction of Potent Ricin-Neutralizing Antibodies.
|
---|---|
Chapter number | 38 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3387-7_38 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3385-3, 978-1-4939-3387-7
|
Authors |
Tamar Sabo, Chanoch Kronman, Ohad Mazor |
Editors |
Sunil Thomas |
Abstract |
Ricin is one of the most potent and lethal toxins known to which there is no available antidote. Currently, the most promising therapy is based on neutralizing antibodies elicited by active vaccination or given passively. Here, detailed protocols are provided for the production of two ricin holotoxin-based vaccines: monomerized subunit-based vaccine, and a formaldehyde-based ricin toxoid vaccine. Both vaccines were found to be stable with no toxic activity reversion even after long-term storage while eliciting high anti-ricin antibody titers possessing a potent neutralizing activity. The use of these vaccines is highly suitable for both the production of sera that can be used in passive protection experiments and immunization aimed to isolate potent anti-ricin monoclonal antibodies. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 33% |
Student > Doctoral Student | 1 | 7% |
Professor | 1 | 7% |
Student > Bachelor | 1 | 7% |
Student > Master | 1 | 7% |
Other | 1 | 7% |
Unknown | 5 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 47% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 13% |
Unknown | 6 | 40% |