Chapter title |
Synthesis of Hapten-Protein Conjugate Vaccines with Reproducible Hapten Densities.
|
---|---|
Chapter number | 39 |
Book title |
Vaccine Design
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3387-7_39 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3385-3, 978-1-4939-3387-7
|
Authors |
Oscar B. Torres, Carl R. Alving, Gary R. Matyas, Torres, Oscar B, Alving, Carl R, Matyas, Gary R |
Editors |
Sunil Thomas |
Abstract |
The ability to prepare hapten-carrier conjugates reproducibly with consistent lot-to-lot hapten densities and protein yields is a critical component of hapten vaccine development. This entails the development of appropriate coupling chemistries that do not cause protein precipitation and the development of methods to quantify hapten density. Recently, extensive efforts have been devoted to design vaccines against drugs of abuse. We describe, herein, a method for conjugation of a morphine-like hapten (MorHap) to tetanus toxoid (TT), which involves conjugation of MorHap to the surface lysines of TT through the N-hydroxysuccinimide portion of a heterobifunctional linker and the subsequent attachment of the thiol on MorHap to the maleimide portion of the cross-linker. Methods are described for the analytical quantification of the hapten density of the conjugates using modified Ellman's test, trinitrobenzenesulfonic acid (TNBS) assay, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). |
X Demographics
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 3 | 25% |
Student > Bachelor | 1 | 8% |
Lecturer | 1 | 8% |
Student > Master | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Other | 0 | 0% |
Unknown | 5 | 42% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 1 | 8% |
Chemical Engineering | 1 | 8% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Unknown | 8 | 67% |