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Development and identification of fully human scFv-Fcs against Staphylococcus aureus

Overview of attention for article published in BMC Immunology, April 2016
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Title
Development and identification of fully human scFv-Fcs against Staphylococcus aureus
Published in
BMC Immunology, April 2016
DOI 10.1186/s12865-016-0146-z
Pubmed ID
Authors

Siji Nian, Tong Wu, Yingchun Ye, Xu Wang, Wenfeng Xu, Qing Yuan

Abstract

Staphylococcus aureus, a gram-positive pathogen, causes many human infections. Methicillin-resistant S. aureus (MRSA) is the most common drug-resistance bacteria. Nearly all MRSA bacteria are resistant to several drugs. Specific antibodies are the main components of the host's humoral immunity, and play a significant role in the process of the host's resistance to bacterial infection. A single-chain variable fragment (scFv) library was constructed using mRNA from the peripheral blood mononuclear cells of S. aureus infected volunteers. After the scFv library DNA was transformed into Escherichia coli TG1, ~1.7 × 10(7) independent clones with full-length scFv inserts. The scFv library was screened by phage display for three rounds using S. aureus as an antigen. The single clones were chosen at random and the scFvs were expressed for enzyme-linked immunosorbent assay (ELISA) assessment. Approximately 50 % of the clones were positive with good binding activity to S. aureus. To improve the stability of scFvs, scFv-fragment crystallizable regions (-Fcs) were constructed and expressed in E. coli DH5α. The expressed scFv-Fcs were purified and identified by western blot. These antibodies were further characterized and analyzed for bioactivity. The results showed that the expression level and folding of scFv-Fcs induced at 25 °C without isopropyl β-D-1-thiogalactopyranoside (IPTG) were higher than that induced at 32 °C with 1.0 mmol/L IPTG. scFv-Fcs had good bioactivity and could specifically bind with S. aureus. scFv-Fcs against S. aureus were successfully constructed and are good candidates for the development of future adjunctive therapy for severe S. aureus infections.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 38 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 21%
Student > Bachelor 6 16%
Student > Doctoral Student 3 8%
Student > Ph. D. Student 3 8%
Student > Master 3 8%
Other 5 13%
Unknown 10 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 24%
Agricultural and Biological Sciences 8 21%
Medicine and Dentistry 3 8%
Chemical Engineering 2 5%
Mathematics 1 3%
Other 3 8%
Unknown 12 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 May 2016.
All research outputs
#20,323,943
of 22,867,327 outputs
Outputs from BMC Immunology
#503
of 588 outputs
Outputs of similar age
#253,365
of 299,065 outputs
Outputs of similar age from BMC Immunology
#7
of 9 outputs
Altmetric has tracked 22,867,327 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 588 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 299,065 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.