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Methods for selection of Daphnia resting eggs: the influence of manual decapsulation and sodium hypoclorite solution on hatching rates

Overview of attention for article published in Brazilian Journal of Biology, May 2016
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Title
Methods for selection of Daphnia resting eggs: the influence of manual decapsulation and sodium hypoclorite solution on hatching rates
Published in
Brazilian Journal of Biology, May 2016
DOI 10.1590/1519-6984.09415
Pubmed ID
Authors

T A S V Paes, A C Rietzler, P M Maia-Barbosa

Abstract

Cladocerans are able to produce resting eggs inside a protective resistant capsule, the ephippium, that difficults the visualization of the resting eggs, because of the dark pigmentation. Therefore, before hatching experiments, methods to verify viable resting eggs in ephippia must be considered. This study aimed to evaluate the number of eggs per ephippium of Daphnia from two tropical aquatic ecosystems and the efficiency of some methods for decapsulating resting eggs. To evaluate the influence of methods on hatching rates, three different conditions were tested: immersion in sodium hypochlorite, manually decapsulated resting eggs and intact ephippia. The immersion in hypochlorite solution could evaluate differences in numbers of resting eggs per ephippium between the ecosystems studied. The exposure to sodium hypochlorite at a concentration of 2% for 20 minutes was the most efficient method for visual evaluation and isolation of the resting eggs. Hatching rate experiments with resting eggs not isolated from ephippia were underestimated (11.1 ± 5.0%), showing the need of methods to quantify and isolate viable eggs. There were no differences between the hatching rate of resting eggs submitted to hypochlorite solution (47.2 ± 7.34%) and manually decapsulated (53.7 ± 13.24%). However, the immersion in hypochlorite was a more efficient technique, faster and not requiring manual ability.

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The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 17%
Researcher 1 4%
Student > Bachelor 1 4%
Unknown 18 75%
Readers by discipline Count As %
Environmental Science 2 8%
Biochemistry, Genetics and Molecular Biology 1 4%
Agricultural and Biological Sciences 1 4%
Immunology and Microbiology 1 4%
Unknown 19 79%