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Detection and quantification of Lyme spirochetes using sensitive and specific molecular beacon probes

Overview of attention for article published in BMC Microbiology, January 2009
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (75th percentile)
  • Good Attention Score compared to outputs of the same age and source (78th percentile)

Mentioned by

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1 patent
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1 research highlight platform

Citations

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13 Dimensions

Readers on

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28 Mendeley
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Title
Detection and quantification of Lyme spirochetes using sensitive and specific molecular beacon probes
Published in
BMC Microbiology, January 2009
DOI 10.1186/1471-2180-9-43
Pubmed ID
Authors

Diana S Saidac, Salvatore A E Marras, Nikhat Parveen

Abstract

Lyme disease, caused by Borrelia burgdorferi, affects a large number of people in both the USA and Europe. The mouse is a natural host for this spirochete and is widely used as a model system to study Lyme pathogenesis mechanisms. Since disease manifestations often depend upon the spirochete burden in a particular tissue, it is critical to accurately measure the bacterial number in infected tissues. The current methods either lack sensitivity and specificity (SYBR Green), or require independent analysis of samples in parallel to quantitate host and bacterial DNA (TaqMan). We have developed a novel molecular beacon-based convenient multiplex real-time quantitative PCR assay to identify and detect small numbers of B. burgdorferi in infected mouse tissues.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 14%
Czechia 1 4%
Unknown 23 82%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 25%
Researcher 7 25%
Student > Master 4 14%
Professor > Associate Professor 3 11%
Student > Postgraduate 2 7%
Other 4 14%
Unknown 1 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 46%
Immunology and Microbiology 4 14%
Biochemistry, Genetics and Molecular Biology 4 14%
Medicine and Dentistry 2 7%
Philosophy 1 4%
Other 3 11%
Unknown 1 4%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 May 2017.
All research outputs
#3,121,883
of 12,372,276 outputs
Outputs from BMC Microbiology
#371
of 1,804 outputs
Outputs of similar age
#27,288
of 117,937 outputs
Outputs of similar age from BMC Microbiology
#3
of 14 outputs
Altmetric has tracked 12,372,276 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 1,804 research outputs from this source. They receive a mean Attention Score of 3.8. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 117,937 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.