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Detection and quantification of Lyme spirochetes using sensitive and specific molecular beacon probes

Overview of attention for article published in BMC Microbiology, February 2009
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (88th percentile)
  • High Attention Score compared to outputs of the same age and source (84th percentile)

Mentioned by

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3 patents
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1 research highlight platform

Citations

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14 Dimensions

Readers on

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35 Mendeley
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Title
Detection and quantification of Lyme spirochetes using sensitive and specific molecular beacon probes
Published in
BMC Microbiology, February 2009
DOI 10.1186/1471-2180-9-43
Pubmed ID
Authors

Diana S Saidac, Salvatore AE Marras, Nikhat Parveen

Abstract

Lyme disease, caused by Borrelia burgdorferi, affects a large number of people in both the USA and Europe. The mouse is a natural host for this spirochete and is widely used as a model system to study Lyme pathogenesis mechanisms. Since disease manifestations often depend upon the spirochete burden in a particular tissue, it is critical to accurately measure the bacterial number in infected tissues. The current methods either lack sensitivity and specificity (SYBR Green), or require independent analysis of samples in parallel to quantitate host and bacterial DNA (TaqMan). We have developed a novel molecular beacon-based convenient multiplex real-time quantitative PCR assay to identify and detect small numbers of B. burgdorferi in infected mouse tissues.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 11%
Czechia 1 3%
Unknown 30 86%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 23%
Researcher 7 20%
Professor > Associate Professor 4 11%
Student > Master 4 11%
Student > Postgraduate 2 6%
Other 5 14%
Unknown 5 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 37%
Biochemistry, Genetics and Molecular Biology 5 14%
Immunology and Microbiology 5 14%
Medicine and Dentistry 3 9%
Psychology 1 3%
Other 3 9%
Unknown 5 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 April 2024.
All research outputs
#3,482,554
of 25,738,558 outputs
Outputs from BMC Microbiology
#298
of 3,514 outputs
Outputs of similar age
#11,762
of 110,443 outputs
Outputs of similar age from BMC Microbiology
#3
of 19 outputs
Altmetric has tracked 25,738,558 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,514 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 110,443 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.