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A cell transportation solution that preserves live circulating tumor cells in patient blood samples

Overview of attention for article published in BMC Cancer, May 2016
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Title
A cell transportation solution that preserves live circulating tumor cells in patient blood samples
Published in
BMC Cancer, May 2016
DOI 10.1186/s12885-016-2330-1
Pubmed ID
Authors

Steingrimur Stefansson, Daniel L. Adams, William B. Ershler, Huyen Le, David H. Ho

Abstract

Circulating tumor cells (CTCs) are typically collected into CellSave fixative tubes, which kills the cells, but preserves their morphology. Currently, the clinical utility of CTCs is mostly limited to their enumeration. More detailed investigation of CTC biology can be performed on live cells, but obtaining live CTCs is technically challenging, requiring blood collection into biocompatible solutions and rapid isolation which limits transportation options. To overcome the instability of CTCs, we formulated a sugar based cell transportation solution (SBTS) that stabilizes cell viability at ambient temperature. In this study we examined the long term viability of human cancer cell lines, primary cells and CTCs in human blood samples in the SBTS for transportation purposes. Four cell lines, 5 primary human cells and purified human PBMCs were tested to determine the viability of cells stored in the transportation solution at ambient temperature for up to 7 days. We then demonstrated viability of MCF-7 cells spiked into normal blood with SBTS and stored for up to 7 days. A pilot study was then run on blood samples from 3 patients with metastatic malignancies stored with or without SBTS for 6 days. CTCs were then purified by Ficoll separation/microfilter isolation and identified using CTC markers. Cell viability was assessed using trypan blue or CellTracker™ live cell stain. Our results suggest that primary/immortalized cell lines stored in SBTS remain ~90 % viable for > 72 h. Further, MCF-7 cells spiked into whole blood remain viable when stored with SBTS for up to 7 days. Finally, live CTCs were isolated from cancer patient blood samples kept in SBTS at ambient temperature for 6 days. No CTCs were isolated from blood samples stored without SBTS. In this proof of principle pilot study we show that viability of cell lines is preserved for days using SBTS. Further, this solution can be used to store patient derived blood samples for eventual isolation of viable CTCs after days of storage. Therefore, we suggest an effective and economical transportation of cancer patient blood samples containing live CTCs can be achieved.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Korea, Republic of 1 3%
United States 1 3%
Unknown 27 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 24%
Student > Ph. D. Student 6 21%
Student > Master 5 17%
Student > Bachelor 3 10%
Student > Doctoral Student 2 7%
Other 2 7%
Unknown 4 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 28%
Medicine and Dentistry 6 21%
Agricultural and Biological Sciences 6 21%
Engineering 2 7%
Nursing and Health Professions 1 3%
Other 2 7%
Unknown 4 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 May 2016.
All research outputs
#6,628,139
of 7,659,635 outputs
Outputs from BMC Cancer
#2,716
of 3,313 outputs
Outputs of similar age
#223,006
of 266,484 outputs
Outputs of similar age from BMC Cancer
#73
of 103 outputs
Altmetric has tracked 7,659,635 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,313 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 103 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.