| Chapter title |
The Nuclear Envelope
|
|---|---|
| Chapter number | 10 |
| Book title |
The Nuclear Envelope
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3530-7_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3528-4, 978-1-4939-3530-7
|
| Authors |
Drozdz, Marek, Vaux, David J, Marek Drozdz, David J. Vaux, Vaux, David J. |
| Editors |
Sue Shackleton, Philippe Collas, Eric C. Schirmer |
| Abstract |
Pulse-chase methods offer powerful tools for following the evolution of a biological system over time, but are usually limited to ensemble measurements of the average behavior of very large numbers of cells. Here we describe three methods ranging from a true pulse-chase, through selective regional photoactivation, to pharmacological induction of an altered protein state, which can be applied to time-dependent studies at the single-cell level. These methods are exemplified by experimental protocols to follow region-selective nuclear envelope targeting of nascent phospholipids, a nascent nuclear lamin protein (lamin B1), and an immature lamin precursor (prelamin A). |
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