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The Nuclear Envelope

Overview of attention for book
Cover of 'The Nuclear Envelope'

Table of Contents

  1. Altmetric Badge
    Book Overview
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    Chapter 1 The Nuclear Envelope
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    Chapter 2 Exploring the Protein Composition of the Plant Nuclear Envelope.
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    Chapter 3 High-Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes.
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    Chapter 4 The Nuclear Envelope
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    Chapter 5 Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS).
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    Chapter 6 The Nuclear Envelope
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    Chapter 7 The Use of Two-Photon FRET-FLIM to Study Protein Interactions During Nuclear Envelope Fusion In Vivo and In Vitro.
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    Chapter 8 Identifying Protein-Protein Associations at the Nuclear Envelope with BioID.
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    Chapter 9 In Situ Detection of Interactions Between Nuclear Envelope Proteins and Partners.
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    Chapter 10 The Nuclear Envelope
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    Chapter 11 Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional Complementation.
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    Chapter 12 The Nuclear Envelope
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    Chapter 13 Identification and Validation of Putative Nesprin Variants.
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    Chapter 14 Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting.
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    Chapter 15 The Use of Polyacrylamide Hydrogels to Study the Effects of Matrix Stiffness on Nuclear Envelope Properties.
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    Chapter 16 Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling.
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    Chapter 17 The Nuclear Envelope
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    Chapter 18 Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells.
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    Chapter 19 The Nuclear Envelope
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    Chapter 20 The Nuclear Envelope
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    Chapter 21 The Nuclear Envelope
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    Chapter 22 The Nuclear Envelope
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    Chapter 23 The Nuclear Envelope
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    Chapter 24 The Nuclear Envelope
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    Chapter 25 The Nuclear Envelope
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    Chapter 26 Methods to Monitor DNA Repair Defects and Genomic Instability in the Context of a Disrupted Nuclear Lamina.
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    Chapter 27 The Nuclear Envelope
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    Chapter 28 An In Vitro Assay to Study Targeting of Membrane Proteins to the Inner Nuclear Membrane.
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    Chapter 29 Nuclear Protein Transport in Digitonin Permeabilized Cells.
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    Chapter 30 Analysis of CRM1-Dependent Nuclear Export in Permeabilized Cells.
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    Chapter 31 The Nuclear Envelope
Attention for Chapter 20: The Nuclear Envelope
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Chapter title
The Nuclear Envelope
Chapter number 20
Book title
The Nuclear Envelope
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3530-7_20
Pubmed ID
Book ISBNs
978-1-4939-3528-4, 978-1-4939-3530-7
Authors

Oldenburg, Anja R, Collas, Philippe, Anja R. Oldenburg, Philippe Collas

Editors

Sue Shackleton, Philippe Collas, Eric C. Schirmer

Abstract

The nuclear lamina is a meshwork of A- and B-type lamins which interact with chromatin and regulate many nuclear functions. Recent studies have reported the discovery of chromatin domains interacting with nuclear lamins by chromatin immunoprecipitation (ChIP) of lamin A/C or B1 and identification of the associated DNA sequences by microarray or high-throughput sequencing. ChIP has been used over many years to get a snapshot of interactions between DNA and proteins in cells, including modified histones, transcription factors, chromatin remodelers, and recently, structural proteins such as nuclear lamins. We describe here the procedure we have established in our laboratory for ChIP of lamin A/C and lamin B1 from human cultured cells. The protocol is compatible with polymerase chain reaction and high-throughput sequencing analysis of the co-immunoprecipitated DNA.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 35%
Student > Doctoral Student 2 10%
Other 2 10%
Researcher 2 10%
Professor > Associate Professor 2 10%
Other 3 15%
Unknown 2 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 45%
Agricultural and Biological Sciences 3 15%
Medicine and Dentistry 3 15%
Psychology 1 5%
Neuroscience 1 5%
Other 1 5%
Unknown 2 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 May 2016.
All research outputs
#6,628,165
of 7,659,635 outputs
Outputs from Methods in molecular biology
#3,850
of 5,430 outputs
Outputs of similar age
#222,894
of 266,664 outputs
Outputs of similar age from Methods in molecular biology
#11
of 17 outputs
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