| Chapter title |
Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling.
|
|---|---|
| Chapter number | 16 |
| Book title |
The Nuclear Envelope
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3530-7_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3528-4, 978-1-4939-3530-7
|
| Authors |
Gregory Fedorchak, Jan Lammerding |
| Editors |
Sue Shackleton, Philippe Collas, Eric C. Schirmer |
| Abstract |
To evaluate the intracellular force transmission between the nucleus and cytoskeleton, we optimized a single cell-based assay that involves the manipulation of living, adherent cells with a fine glass microneedle and a microscope-mounted micromanipulator. The user inserts the microneedle into the cytoplasm and then, using a custom-programmable computer script, pulls the needle laterally toward the cell periphery. Normalized cross-correlation is applied to recorded time-lapse image sequences to determine average displacements within predefined regions of the nucleus and the cytoskeleton. These regional displacements, together with calculations of nuclear elongation, nuclear centroid translocation, and nuclear shape changes, enable quantitative assessments of nucleo-cytoskeletal coupling in both normal and disease conditions and provide an improved understanding of the role of specific nuclear envelope proteins in intracellular force propagation. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | 20% |
| Japan | 1 | 5% |
| Singapore | 1 | 5% |
| Austria | 1 | 5% |
| Brazil | 1 | 5% |
| Germany | 1 | 5% |
| United Kingdom | 1 | 5% |
| Canada | 1 | 5% |
| India | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 8 | 40% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 13 | 65% |
| Scientists | 6 | 30% |
| Practitioners (doctors, other healthcare professionals) | 1 | 5% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 30 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 23% |
| Researcher | 6 | 20% |
| Student > Master | 4 | 13% |
| Student > Bachelor | 3 | 10% |
| Student > Doctoral Student | 1 | 3% |
| Other | 5 | 17% |
| Unknown | 4 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 43% |
| Agricultural and Biological Sciences | 4 | 13% |
| Physics and Astronomy | 2 | 7% |
| Unspecified | 1 | 3% |
| Chemical Engineering | 1 | 3% |
| Other | 3 | 10% |
| Unknown | 6 | 20% |