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Recombinant raccoon pox vaccine protects mice against lethal plague

Overview of attention for article published in Vaccine, March 2003
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (86th percentile)
  • High Attention Score compared to outputs of the same age and source (81st percentile)

Mentioned by

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4 patents
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2 Wikipedia pages

Readers on

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27 Mendeley
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Title
Recombinant raccoon pox vaccine protects mice against lethal plague
Published in
Vaccine, March 2003
DOI 10.1016/s0264-410x(02)00557-1
Pubmed ID
Authors

Jorge E Osorio, Tim D Powell, Rexann S Frank, Kelley Moss, Elizabeth J Haanes, Susan R Smith, Tonie E Rocke, Dan T Stinchcomb

Abstract

Using a raccoon poxvirus (RCN) expression system, we have developed new recombinant vaccines that can protect mice against lethal plague infection. We tested the effects of a translation enhancer (EMCV-IRES) in combination with a secretory (tPA) signal or secretory (tPA) and membrane anchoring (CHV-gG) signals on in vitro antigen expression of F1 antigen in tissue culture and the induction of antibody responses and protection against Yersinia pestis challenge in mice. The RCN vector successfully expressed the F1 protein of Y. pestis in vitro. In addition, the level of expression was increased by the insertion of the EMCV-IRES and combinations of this and the secretory signal or secretory and anchoring signals. These recombinant viruses generated protective immune responses that resulted in survival of 80% of vaccinated mice upon challenge with Y. pestis. Of the RCN-based vaccines we tested, the RCN-IRES-tPA-YpF1 recombinant construct was the most efficacious. Mice vaccinated with this construct withstood challenge with as many as 1.5 million colony forming units of Y. pestis (7.7 x 10(4)LD(50)). Interestingly, vaccination with F1 fused to the anchoring signal (RCN-IRES-tPA-YpF1-gG) elicited significant anti-F1 antibody titers, but failed to protect mice from plague challenge. Our studies demonstrate, in vitro and in vivo, the potential importance of the EMCV-IRES and secretory signals in vaccine design. These molecular tools provide a new approach for improving the efficacy of vaccines. In addition, these novel recombinant vaccines could have human, veterinary, and wildlife applications in the prevention of plague.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 4%
United States 1 4%
Unknown 25 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 37%
Other 5 19%
Student > Ph. D. Student 5 19%
Student > Bachelor 2 7%
Professor > Associate Professor 2 7%
Other 2 7%
Unknown 1 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 44%
Veterinary Science and Veterinary Medicine 6 22%
Medicine and Dentistry 3 11%
Environmental Science 1 4%
Immunology and Microbiology 1 4%
Other 3 11%
Unknown 1 4%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 August 2017.
All research outputs
#3,798,287
of 25,373,627 outputs
Outputs from Vaccine
#3,259
of 16,509 outputs
Outputs of similar age
#5,833
of 62,542 outputs
Outputs of similar age from Vaccine
#12
of 96 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 16,509 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 17.7. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 62,542 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 96 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.