Natália Yoshie Kawakami, Fernanda Tomiotto-Pellissier, Allan Henrique Depieri Cataneo, Tatiane Marcusso Orsini, Ana Paula Fortes Dos Santos Thomazelli, Carolina Panis, Ivete Conchon-Costa, Wander Rogério Pavanelli
Abstract
Leishmaniasis is a zoonotic disease caused by protozoa of the genus Leishmania . Cutaneous leishmaniasis is the most common form, with millions of new cases worldwide each year. Treatments are ineffective due to the toxicity of existing drugs and the resistance acquired by certain strains of the parasite.
We evaluated the activity of sodium nitroprusside in macrophages infected with Leishmania (Leishmania) amazonensis . Phagocytic and microbicidal activity were evaluated by phagocytosis assay and promastigote recovery, respectively, while cytokine production and nitrite levels were determined by ELISA and by the Griess method. Levels of iNOS and 3-nitrotyrosine were measured by immunocytochemistry.
Sodium nitroprusside exhibited in vitro antileishmanial activity at both concentrations tested, reducing the number of amastigotes and recovered promastigotes in macrophages infected with L. amazonensis . At 1.5µg/mL, sodium nitroprusside stimulated levels of TNF-α and nitric oxide, but not IFN-γ. The compound also increased levels of 3-nitrotyrosine, but not expression of iNOS, suggesting that the drug acts as an exogenous source of nitric oxide.
Sodium nitroprusside enhances microbicidal activity in Leishmania -infected macrophages by boosting nitric oxide and 3-nitrotyrosine.
Pharmacology, Toxicology and Pharmaceutical Science
1
4%
Other
2
9%
Unknown
8
35%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 May 2016.
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#22,758,309
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Outputs from Revista da Sociedade Brasileira de Medicina Tropical
#953
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#347,796
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Outputs of similar age from Revista da Sociedade Brasileira de Medicina Tropical
#9
of 12 outputs
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