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Allelic sequence heterozygosity in single Giardia parasites

Overview of attention for article published in BMC Microbiology, May 2012
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Title
Allelic sequence heterozygosity in single Giardia parasites
Published in
BMC Microbiology, May 2012
DOI 10.1186/1471-2180-12-65
Pubmed ID
Authors

Johan Ankarklev, Staffan G Svärd, Marianne Lebbad

Abstract

Genetic heterogeneity has become a major inconvenience in the genotyping and molecular epidemiology of the intestinal protozoan parasite Giardia intestinalis, in particular for the major human infecting genotype, assemblage B. Sequence-based genotyping of assemblage B Giardia from patient fecal samples, where one or several of the commonly used genotyping loci (beta-giardin, triosephosphate isomerase and glutamate dehydrogenase) are implemented, is often hampered due to the presence of sequence heterogeneity in the sequencing chromatograms. This can be due to allelic sequence heterozygosity (ASH) and /or co-infections with parasites of different assemblage B sub-genotypes. Thus, two important questions have arisen; i) does ASH occur at the single cell level, and/or ii) do multiple sub-genotype infections commonly occur in patients infected with assemblage B, G. intestinalis isolates?

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Norway 1 2%
Peru 1 2%
Argentina 1 2%
Unknown 42 91%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 17%
Student > Bachelor 7 15%
Student > Ph. D. Student 7 15%
Other 5 11%
Professor > Associate Professor 4 9%
Other 8 17%
Unknown 7 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 35%
Biochemistry, Genetics and Molecular Biology 9 20%
Immunology and Microbiology 5 11%
Veterinary Science and Veterinary Medicine 4 9%
Medicine and Dentistry 2 4%
Other 2 4%
Unknown 8 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 June 2012.
All research outputs
#20,656,161
of 25,374,647 outputs
Outputs from BMC Microbiology
#2,468
of 3,489 outputs
Outputs of similar age
#137,672
of 175,882 outputs
Outputs of similar age from BMC Microbiology
#25
of 30 outputs
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