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The nitrogen-fixing gene (nifH) of Rhodopseudomonas palustris: a case of lateral gene transfer?

Overview of attention for article published in Microbiology, July 2004
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Title
The nitrogen-fixing gene (nifH) of Rhodopseudomonas palustris: a case of lateral gene transfer?
Published in
Microbiology, July 2004
DOI 10.1099/mic.0.26940-0
Pubmed ID
Authors

Jose Jason L. Cantera, Hiroko Kawasaki, Tatsuji Seki

Abstract

Nitrogen fixation is catalysed by some photosynthetic bacteria. This paper presents a phylogenetic comparison of a nitrogen fixation gene (nifH) with the aim of elucidating the processes underlying the evolutionary history of Rhodopseudomonas palustris. In the NifH phylogeny, strains of Rps. palustris were placed in close association with Rhodobacter spp. and other phototrophic purple non-sulfur bacteria belonging to the alpha-Proteobacteria, separated from its close relatives Bradyrhizobium japonicum and the phototrophic rhizobia (Bradyrhizobium spp. IRBG 2, IRBG 228, IRBG 230 and BTAi 1) as deduced from the 16S rRNA phylogeny. The close association of the strains of Rps. palustris with those of Rhodobacter and Rhodovulum, as well as Rhodospirillum rubrum, was supported by the mol% G+C of their nifH gene and by the signature sequences found in the sequence alignment. In contrast, comparison of a number of informational and operational genes common to Rps. palustris CGA009, B. japonicum USDA 110 and Rhodobacter sphaeroides 2.4.1 suggested that the genome of Rps. palustris is more related to that of B. japonicum than to the Rba. sphaeroides genome. These results strongly suggest that the nifH of Rps. palustris is highly related to those of the phototrophic purple non-sulfur bacteria included in this study, and might have come from an ancestral gene common to these phototrophic species through lateral gene transfer. Although this finding complicates the use of nifH to infer the phylogenetic relationships among the phototrophic bacteria in molecular diversity studies, it establishes a framework to resolve the origins and diversification of nitrogen fixation among the phototrophic bacteria in the alpha-Proteobacteria.

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Mendeley readers

The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 3%
Brazil 2 3%
New Zealand 1 2%
Canada 1 2%
United Kingdom 1 2%
Egypt 1 2%
Mexico 1 2%
Russia 1 2%
Italy 1 2%
Other 0 0%
Unknown 47 81%

Demographic breakdown

Readers by professional status Count As %
Researcher 21 36%
Student > Ph. D. Student 14 24%
Student > Doctoral Student 7 12%
Student > Master 4 7%
Lecturer 3 5%
Other 8 14%
Unknown 1 2%
Readers by discipline Count As %
Agricultural and Biological Sciences 42 72%
Biochemistry, Genetics and Molecular Biology 4 7%
Chemistry 2 3%
Environmental Science 2 3%
Earth and Planetary Sciences 1 2%
Other 3 5%
Unknown 4 7%