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In vivo imaging of Lactococcus lactis, Lactobacillus plantarum and Escherichia coli expressing infrared fluorescent protein in mice

Overview of attention for article published in Microbial Cell Factories, November 2015
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Title
In vivo imaging of Lactococcus lactis, Lactobacillus plantarum and Escherichia coli expressing infrared fluorescent protein in mice
Published in
Microbial Cell Factories, November 2015
DOI 10.1186/s12934-015-0376-4
Pubmed ID
Authors

Aleš Berlec, Janja Završnik, Miha Butinar, Boris Turk, Borut Štrukelj

Abstract

In vivo imaging of orally administered lactic acid bacteria (LAB) and commensal bacteria in mice is shown to provide information on the spatial and temporal distribution of bacteria in the gastrointestinal tract. The bacteria can be detected and monitored using bioluminescence or near-infrared fluorescence. Fluorescence imaging of bacteria was established by expressing the infrared fluorescent protein IRFP713 in Lactococcus lactis, Lactobacillus plantarum and Escherichia coli. All three bacterial species were monitored in live mice and no major differences in transit time were observed. Bacteria passed through the stomach and small intestine in 1 h and the majority were secreted from the large intestine after 6-8 h. Intestinal localization of bacteria was confirmed by imaging the isolated intestines and culturing the intestinal content. The use of fluorescence tomography for spatial localization of fluorescent bacteria has been established. The expression of an additional infrared fluorescent protein IRFP682 enabled concomitant detection of two bacterial populations in live mice. The present work provides a methodological basis for future studies of probiotic and theranostic actions of LAB in mouse disease models.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 73 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 1%
Unknown 72 99%

Demographic breakdown

Readers by professional status Count As %
Researcher 20 27%
Student > Ph. D. Student 15 21%
Student > Master 9 12%
Student > Bachelor 5 7%
Other 4 5%
Other 9 12%
Unknown 11 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 22 30%
Agricultural and Biological Sciences 12 16%
Immunology and Microbiology 9 12%
Engineering 6 8%
Medicine and Dentistry 5 7%
Other 5 7%
Unknown 14 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 May 2016.
All research outputs
#15,373,286
of 22,870,727 outputs
Outputs from Microbial Cell Factories
#988
of 1,604 outputs
Outputs of similar age
#164,287
of 281,550 outputs
Outputs of similar age from Microbial Cell Factories
#20
of 36 outputs
Altmetric has tracked 22,870,727 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,604 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 281,550 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.