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Novel strong promoter of antimicrobial peptides gene pro-SmAMP2 from chickweed (Stellaria media)

Overview of attention for article published in BMC Biotechnology, May 2016
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Title
Novel strong promoter of antimicrobial peptides gene pro-SmAMP2 from chickweed (Stellaria media)
Published in
BMC Biotechnology, May 2016
DOI 10.1186/s12896-016-0273-x
Pubmed ID
Authors

Roman A. Komakhin, Denis A. Vysotskii, Rahim R. Shukurov, Vera D. Voblikova, Vera V. Komakhina, Svetlana R. Strelnikova, Ekaterina M. Vetchinkina, Alexey V. Babakov

Abstract

In a previous study we found that in chickweed the expression level of the pro-SmAMP2 gene was comparable or even higher to that of the β-actin gene. This high level of the gene expression has attracted our attention as an opportunity for the identification of novel strong promoters of plant origin, which could find its application in plant biotechnology. Therefore, in the present study we focused on the nucleotide sequence identification and the functional characteristics of the pro-SmAMP2 promoter in transgenic plants. In chickweed (Stellaria media), a 2120 bp promoter region of the pro-SmAMP2 gene encoding antifungal peptides was sequenced. Six 5'-deletion variants -2120, -1504, -1149, -822, -455, and -290 bp of pro-SmAMP2 gene promoter were fused with the coding region of the reporter gene gusA in the plant expression vector pCambia1381Z. Independent transgenic plants of tobacco Nicotiana tabacum were obtained with each genetic structure. GUS protein activity assay in extracts from transgenic plants showed that all deletion variants of the promoter, except -290 bp, expressed the gusA gene. In most transgenic plants, the GUS activity level was comparable or higher than in plants with the viral promoter CaMV 35S. GUS activity remains high in progenies and its level correlates positively with the amount of gusA gene mRNA in T3 homozygous plants. The activity of the рro-SmAMP2 promoter was detected in all organs of the transgenic plants studied, during meiosis and in pollen as well. Our results show that the рro-SmAMP2 promoter can be used for target genes expression control in transgenic plants.

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Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 30%
Student > Master 1 10%
Student > Ph. D. Student 1 10%
Researcher 1 10%
Student > Postgraduate 1 10%
Other 0 0%
Unknown 3 30%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 30%
Immunology and Microbiology 1 10%
Nursing and Health Professions 1 10%
Medicine and Dentistry 1 10%
Chemistry 1 10%
Other 0 0%
Unknown 3 30%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 May 2016.
All research outputs
#5,833,673
of 7,715,105 outputs
Outputs from BMC Biotechnology
#354
of 468 outputs
Outputs of similar age
#188,294
of 269,129 outputs
Outputs of similar age from BMC Biotechnology
#17
of 22 outputs
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So far Altmetric has tracked 468 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
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We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.