| Chapter title |
Plant Proteostasis
|
|---|---|
| Chapter number | 13 |
| Book title |
Plant Proteostasis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3759-2_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3757-8, 978-1-4939-3759-2
|
| Authors |
Pu, Yunting, Bassham, Diane C, Yunting Pu, Diane C. Bassham |
| Editors |
L. Maria Lois, Rune Matthiesen |
| Abstract |
Autophagy is a key process for degradation and recycling of proteins or organelles in eukaryotes. Autophagy in plants has been shown to function in stress responses, pathogen immunity, and senescence, while a basal level of autophagy plays a housekeeping role in cells. Upon activation of autophagy, vesicles termed autophagosomes are formed to deliver proteins or organelles to the vacuole for degradation. The number of autophagosomes can thus be used to indicate the level of autophagy. Here, we describe two common methods used for detection of autophagosomes, staining of autophagosomes with the fluorescent dye monodansylcadaverine, and expression of a fusion between GFP and the autophagosomal membrane protein ATG8. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 24% |
| Other | 4 | 19% |
| Student > Master | 2 | 10% |
| Student > Ph. D. Student | 2 | 10% |
| Student > Doctoral Student | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 4 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 48% |
| Biochemistry, Genetics and Molecular Biology | 4 | 19% |
| Nursing and Health Professions | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Unknown | 5 | 24% |