| Chapter title |
Fluorescent Reporters for Ubiquitin-Dependent Proteolysis in Plants
|
|---|---|
| Chapter number | 5 |
| Book title |
Plant Proteostasis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3759-2_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3757-8, 978-1-4939-3759-2
|
| Authors |
Katarzyna Zientara-Rytter, Agnieszka Sirko |
| Editors |
L. Maria Lois, Rune Matthiesen |
| Abstract |
Ubiquitin is a small protein commonly used as a signal molecule which upon attachment to the proteins affects their function and their fate in the cells. For example, it can be used as a degradation marker by the cell. Ubiquitin plays a significant role in regulation of numerous cellular processes. Therefore, monitoring of ubiquitin-dependent proteolysis can provide important information. Here, we describe construction of YFP-based proteasome substrates containing modified ubiquitin and the protocol for their transient expression in plant cells for functional analysis of the ubiquitin/proteasome system. To facilitate further subcloning all plasmids generated by us are based on the Gateway(®) Cloning Technology and are compatible with the Gateway(®) destination vectors. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 40% |
| Unspecified | 1 | 20% |
| Professor > Associate Professor | 1 | 20% |
| Other | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 40% |
| Unspecified | 1 | 20% |
| Agricultural and Biological Sciences | 1 | 20% |
| Unknown | 1 | 20% |