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Plant Proteostasis

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Cover of 'Plant Proteostasis'

Table of Contents

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    Book Overview
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    Chapter 1 Approaches to Determine Protein Ubiquitination Residue Types
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    Chapter 2 Immunoprecipitation of Cullin-RING Ligases (CRLs) in Arabidopsis thaliana Seedlings
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    Chapter 3 Radioligand Binding Assays for Determining Dissociation Constants of Phytohormone Receptors
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    Chapter 4 Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes
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    Chapter 5 Fluorescent Reporters for Ubiquitin-Dependent Proteolysis in Plants
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    Chapter 6 Generation of Artificial N-end Rule Substrate Proteins In Vivo and In Vitro
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    Chapter 7 Plant Proteostasis
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    Chapter 8 Plant Proteostasis
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    Chapter 9 Kinetic Analysis of Plant SUMO Conjugation Machinery
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    Chapter 10 Expression, Purification, and Enzymatic Analysis of Plant SUMO Proteases
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    Chapter 11 Quantitative Analysis of Subcellular Distribution of the SUMO Conjugation System by Confocal Microscopy Imaging
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    Chapter 12 Plant Proteostasis
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    Chapter 13 Plant Proteostasis
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    Chapter 14 Protocols for Studying Protein Stability in an Arabidopsis Protoplast Transient Expression System
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    Chapter 15 Detection and Quantification of Protein Aggregates in Plants.
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    Chapter 16 Determination of Protein Carbonylation and Proteasome Activity in Seeds
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    Chapter 17 Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)-Based Protein Profiling in Plants
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    Chapter 18 Plant Proteostasis
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    Chapter 19 In Vivo Radiolabeling of Arabidopsis Chloroplast Proteins and Separation of Thylakoid Membrane Complexes by Blue Native PAGE
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    Chapter 20 Normalized Quantitative Western Blotting Based on Standardized Fluorescent Labeling
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    Chapter 21 Sequence Search and Comparative Genomic Analysis of SUMO-Activating Enzymes Using CoGe
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    Chapter 22 Plant Proteostasis
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    Chapter 23 Bioinformatics Tools for Exploring the SUMO Gene Network
Attention for Chapter 1: Approaches to Determine Protein Ubiquitination Residue Types
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Chapter title
Approaches to Determine Protein Ubiquitination Residue Types
Chapter number 1
Book title
Plant Proteostasis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3759-2_1
Pubmed ID
Book ISBNs
978-1-4939-3757-8, 978-1-4939-3759-2
Authors

Qian Chen, Xiaoyuan Yang, Qi Xie

Editors

L. Maria Lois, Rune Matthiesen

Abstract

Ubiquitination is an important posttranslational modification in eukaryotic organisms and plays a central role in many signaling pathways in plants. Most ubiquitination typically occurs on substrate lysine residues, forming a covalent isopeptide bond. Some recent reports suggested ubiquitin can be attached to non-lysine sites such as serine/threonine, cysteine or the N-terminal methionine, via oxyester or thioester linkages, respectively. In the present protocol, we developed a convenient in vitro assay for investigating ubiquitination on Ser/Thr and Cys residues.

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Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 33%
Other 1 33%
Unknown 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Agricultural and Biological Sciences 1 33%
Unknown 1 33%