The GPCR Consortium,
For all you structural biologists out there! https://link.springer.com/protocol/10.1007%2F978-1-0716-0373-4_8
Expression, Purification, and Structural Biology of Membrane Proteins
Overview of attention for book
Table of Contents
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Altmetric BadgeBook Overview
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Altmetric BadgeChapter 1 Cloning and Multi-Subunit Expression of Mitochondrial Membrane Protein Complexes in Saccharomyces cerevisiae
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Altmetric BadgeChapter 2 Membrane Protein Production in Escherichia coli
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Altmetric BadgeChapter 3 Membrane Protein Production in Lactococcus lactis for Structural Studies
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Altmetric BadgeChapter 6 Membrane Extracts from Plant Tissues
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Altmetric BadgeChapter 8 GPCR Solubilization and Quality Control
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Altmetric BadgeChapter 10 Purification of Membrane Proteins by Affinity Chromatography with On-Column Protease Cleavage
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Altmetric BadgeChapter 11 Biotinylation of Membrane Proteins for Binder Selections
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Altmetric BadgeChapter 13 Identifying Conformation-Selective Heavy-Chain-Only Antibodies Against Membrane Proteins by a Thermal-Shift Scintillation Proximity Assay
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Altmetric BadgeChapter 16 Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent
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Altmetric BadgeChapter 17 Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs
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Altmetric BadgeChapter 18 Fast Small-Scale Membrane Protein Purification and Grid Preparation for Single-Particle Electron Microscopy
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Altmetric BadgeChapter 19 Stabilization and Crystallization of a Membrane Protein Involved in Lipid Transport
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Altmetric BadgeChapter 20 In Meso In Situ Serial X-Ray Crystallography (IMISX): A Protocol for Membrane Protein Structure Determination at the Swiss Light Source
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Altmetric BadgeChapter 21 Membrane Protein Preparation for Serial Crystallography Using High-Viscosity Injectors: Rhodopsin as an Example
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Altmetric BadgeChapter 24 Sample Preparation and Technical Setup for NMR Spectroscopy with Integral Membrane Proteins