| Chapter title |
Recursive construction of perfect DNA molecules and libraries from imperfect oligonucleotides.
|
|---|---|
| Chapter number | 12 |
| Book title |
Gene Synthesis
|
| Published in |
Methods in molecular biology, February 2012
|
| DOI | 10.1007/978-1-61779-564-0_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-563-3, 978-1-61779-564-0
|
| Authors |
Linshiz G, Yehezkel TB, Shapiro E, Gregory Linshiz, Tuval Ben Yehezkel, Ehud Shapiro, Linshiz, Gregory, Yehezkel, Tuval Ben, Shapiro, Ehud |
| Abstract |
Making faultless complex objects from potentially faulty building blocks is a fundamental challenge in computer engineering, nanotechnology, and synthetic biology. We developed an error-correcting recursive construction procedure that attempts to address this challenge. Making DNA molecules from synthetic oligonucleotides using the procedure described here surpasses existing methods for de novo DNA synthesis in speed, precision, and amenability to automation. It provides for the first time a unified DNA construction platform for combining synthetic and natural DNA fragments, for constructing designer DNA libraries, and for making the faultless long synthetic DNA building blocks needed for de novo genome construction. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 6% |
| Australia | 1 | 6% |
| Unknown | 15 | 88% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 24% |
| Student > Ph. D. Student | 4 | 24% |
| Student > Master | 3 | 18% |
| Professor | 2 | 12% |
| Other | 1 | 6% |
| Other | 3 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 82% |
| Biochemistry, Genetics and Molecular Biology | 1 | 6% |
| Unknown | 2 | 12% |