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Gene Synthesis

Overview of attention for book
Cover of 'Gene Synthesis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Building block synthesis using the polymerase chain assembly method.
  3. Altmetric Badge
    Chapter 2 Oligonucleotide assembly in yeast to produce synthetic DNA fragments.
  4. Altmetric Badge
    Chapter 3 TopDown Real-Time Gene Synthesis
  5. Altmetric Badge
    Chapter 4 De Novo DNA Synthesis Using Single-Molecule PCR
  6. Altmetric Badge
    Chapter 5 SLIC: A Method for Sequence- and Ligation-Independent Cloning.
  7. Altmetric Badge
    Chapter 6 Assembly of Standardized DNA Parts Using BioBrick Ends in E. coli.
  8. Altmetric Badge
    Chapter 7 Assembling DNA Fragments by USER Fusion
  9. Altmetric Badge
    Chapter 8 Fusion PCR via Novel Overlap Sequences
  10. Altmetric Badge
    Chapter 9 Using recombineering to generate point mutations: the oligonucleotide-based "hit and fix" method.
  11. Altmetric Badge
    Chapter 10 Using Recombineering to Generate Point Mutations:galK-Based Positive-Negative Selection Method.
  12. Altmetric Badge
    Chapter 11 Assembling Large DNA Segments in Yeast
  13. Altmetric Badge
    Chapter 12 Recursive construction of perfect DNA molecules and libraries from imperfect oligonucleotides.
  14. Altmetric Badge
    Chapter 13 Cloning whole bacterial genomes in yeast.
  15. Altmetric Badge
    Chapter 14 Production of infectious poliovirus from synthetic viral genomes.
  16. Altmetric Badge
    Chapter 15 In silico design of functional DNA constructs.
  17. Altmetric Badge
    Chapter 16 Using DNAWorks in Designing Oligonucleotides for PCR-Based Gene Synthesis
  18. Altmetric Badge
    Chapter 17 De Novo Gene Synthesis Design Using TmPrime Software
  19. Altmetric Badge
    Chapter 18 Design-A-Gene with GeneDesign.
  20. Altmetric Badge
    Chapter 19 Leading a Successful iGEM Team.
  21. Altmetric Badge
    Chapter 20 The Build-a-Genome Course.
  22. Altmetric Badge
    Chapter 21 DNA Synthesis Security
Attention for Chapter 1: Building block synthesis using the polymerase chain assembly method.
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (88th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

Mentioned by

news
1 news outlet
twitter
1 X user

Citations

dimensions_citation
10 Dimensions

Readers on

mendeley
15 Mendeley
citeulike
2 CiteULike
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Chapter title
Building block synthesis using the polymerase chain assembly method.
Chapter number 1
Book title
Gene Synthesis
Published in
Methods in molecular biology, February 2012
DOI 10.1007/978-1-61779-564-0_1
Pubmed ID
Book ISBNs
978-1-61779-563-3, 978-1-61779-564-0
Authors

Julie A. Marchand, Jean Peccoud, Marchand, Julie A., Peccoud, Jean

Abstract

De novo gene synthesis allows the creation of custom DNA molecules without the typical constraints of traditional cloning assembly: scars, restriction site incompatibility, and the quest to find all the desired parts to name a few. Moreover, with the help of computer-assisted design, the perfect DNA molecule can be created along with its matching sequence ready to download. The challenge is to build the physical DNA molecules that have been designed with the software. Although there are several DNA assembly methods, this section presents and describes a method using the polymerase chain assembly (PCA).

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 7%
Unknown 14 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 33%
Professor 3 20%
Student > Master 2 13%
Researcher 2 13%
Student > Bachelor 1 7%
Other 2 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 47%
Biochemistry, Genetics and Molecular Biology 3 20%
Chemical Engineering 2 13%
Unspecified 1 7%
Medicine and Dentistry 1 7%
Other 0 0%
Unknown 1 7%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 March 2014.
All research outputs
#3,099,561
of 22,679,690 outputs
Outputs from Methods in molecular biology
#691
of 13,039 outputs
Outputs of similar age
#27,221
of 250,762 outputs
Outputs of similar age from Methods in molecular biology
#41
of 461 outputs
Altmetric has tracked 22,679,690 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,039 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 250,762 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 461 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.