Title |
PCR detection of malaria parasites in desiccated Anopheles mosquitoes is uninhibited by storage time and temperature
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Published in |
Malaria Journal, June 2012
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DOI | 10.1186/1475-2875-11-193 |
Pubmed ID | |
Authors |
Mark A Rider, Brian D Byrd, Joseph Keating, Dawn M Wesson, Kevin A Caillouet |
Abstract |
Reliable methods to preserve mosquito vectors for malaria studies are necessary for detecting Plasmodium parasites. In field settings, however, maintaining a cold chain of storage from the time of collection until laboratory processing, or accessing other reliable means of sample preservation is often logistically impractical or cost prohibitive. As the Plasmodium infection rate of Anopheles mosquitoes is a central component of the entomological inoculation rate and other indicators of transmission intensity, storage conditions that affect pathogen detection may bias malaria surveillance indicators. This study investigated the effect of storage time and temperature on the ability to detect Plasmodium parasites in desiccated Anopheles mosquitoes by real-time polymerase chain reaction (PCR). |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
United Kingdom | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Indonesia | 1 | 1% |
Australia | 1 | 1% |
Brazil | 1 | 1% |
United Kingdom | 1 | 1% |
United States | 1 | 1% |
Unknown | 83 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 21 | 24% |
Student > Master | 15 | 17% |
Researcher | 14 | 16% |
Student > Bachelor | 8 | 9% |
Other | 5 | 6% |
Other | 12 | 14% |
Unknown | 13 | 15% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 42 | 48% |
Medicine and Dentistry | 9 | 10% |
Biochemistry, Genetics and Molecular Biology | 6 | 7% |
Immunology and Microbiology | 6 | 7% |
Environmental Science | 4 | 5% |
Other | 8 | 9% |
Unknown | 13 | 15% |