↓ Skip to main content

Diverse Burkholderia Species Isolated from Soils in the Southern United States with No Evidence of B. pseudomallei

Overview of attention for article published in PLOS ONE, November 2015
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age

Mentioned by

f1000
1 research highlight platform

Citations

dimensions_citation
17 Dimensions

Readers on

mendeley
48 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Diverse Burkholderia Species Isolated from Soils in the Southern United States with No Evidence of B. pseudomallei
Published in
PLOS ONE, November 2015
DOI 10.1371/journal.pone.0143254
Pubmed ID
Authors

Carina M. Hall, Joseph D. Busch, Kenzie Shippy, Christopher J. Allender, Mirjam Kaestli, Mark Mayo, Jason W. Sahl, James M. Schupp, Rebecca E. Colman, Paul Keim, Bart J. Currie, David M. Wagner

Abstract

The global distribution of the soil-dwelling bacterium Burkholderia pseudomallei, causative agent of melioidosis, is poorly understood. We used established culturing methods developed for B. pseudomallei to isolate Burkholderia species from soil collected at 18 sampling sites in three states in the southern United States (Arizona (n = 4), Florida (n = 7), and Louisiana (n = 7)). Using multi-locus sequence typing (MLST) of seven genes, we identified 35 Burkholderia isolates from these soil samples. All species belonged to the B. cepacia complex (Bcc), including B. cenocepacia, B. cepacia, B. contaminans, B. diffusa, B. metallica, B. seminalis, B. vietnamiensis and two unnamed members of the Bcc. The MLST analysis provided a high level of resolution among and within these species. Despite previous clinical cases within the U.S. involving B. pseudomallei and its close phylogenetic relatives, we did not isolate any of these taxa. The Bcc contains a number of opportunistic pathogens that cause infections in cystic fibrosis patients. Interestingly, we found that B. vietnamiensis was present in soil from all three states, suggesting it may be a common component in southern U.S. soils. Most of the Burkholderia isolates collected in this study were from Florida (30/35; 86%), which may be due to the combination of relatively moist, sandy, and acidic soils found there compared to the other two states. We also investigated one MLST gene, recA, for its ability to identify species within Burkholderia. A 365bp fragment of recA recovered nearly the same species-level identification as MLST, thus demonstrating its cost effective utility when conducting environmental surveys for Burkholderia. Although we did not find B. pseudomallei, our findings document that other diverse Burkholderia species are present in soils in the southern United States.

Mendeley readers

The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Belgium 1 2%
Unknown 46 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 25%
Student > Master 8 17%
Researcher 6 13%
Student > Doctoral Student 3 6%
Student > Bachelor 2 4%
Other 8 17%
Unknown 9 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 25%
Biochemistry, Genetics and Molecular Biology 11 23%
Medicine and Dentistry 5 10%
Chemistry 2 4%
Immunology and Microbiology 2 4%
Other 4 8%
Unknown 12 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 May 2016.
All research outputs
#3,992,172
of 7,705,951 outputs
Outputs from PLOS ONE
#62,992
of 109,477 outputs
Outputs of similar age
#145,796
of 268,513 outputs
Outputs of similar age from PLOS ONE
#2,988
of 4,718 outputs
Altmetric has tracked 7,705,951 research outputs across all sources so far. This one is in the 28th percentile – i.e., 28% of other outputs scored the same or lower than it.
So far Altmetric has tracked 109,477 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.3. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 268,513 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 4,718 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.